Sökning: "Hydrophobic tag"

Visar resultat 6 - 10 av 17 avhandlingar innehållade orden Hydrophobic tag.

1. 6. Outer Coordination Spheres Engineering of Ru-based Molecular Water Oxidation Catalysts

   Författare :Tianqi Liu; Licheng Sun; Julio Lloret-Fillol; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; solar fuels; water oxidation; catalysis; ruthenium complex; Ru-bda; Ru-pda; outer coordination sphere; hydrophobic interactions; Kemi; Chemistry;

   Sammanfattning : The outer coordination sphere plays a vital role in metalloenzyme catalysis, while this principle is relatively less considered in the design of man-made molecular catalysts. This thesis investigates the role of outer coordination spheres in water oxidation by a series of ruthenium-based complexes. LÄS MER

3. 7. The Membrane-Spanning Domain of Complex I Investigated with Fusion Protein Techniques

   Författare :Maria Trane; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NuoN; NuoM; NuoL; covalent bound heme; Bacillus subtilis; Escherichia coli; membrane protein; His-tag; Cytochrome c; NADH:quinone oxidureductase; fusion protein;

   Sammanfattning : NADH:quinone oxidoreductase, or complex I, is a large, complex and poorly understood bioenergetic enzyme in the respiratory chain of living organisms. The enzyme has a conserved core structure, comprising fourteen protein subunits: Seven subunits protrude from the membrane and contain a flavin prosthetic group and eight iron-sulfur clusters that guide electrons from the oxidation of NADH towards the quinone binding site(s). LÄS MER

4. 8. A radical view of haemoglobin - protein engineering and characterisation

   Författare :Marie Grey; Tillämpad biokemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; His-Gln tagging; protein purification; blood substitutes; radical scavenging; superoxide dismutase; E. coli; diglobin; Haemoglobin; protein engineering; aqueous two-phase systems; hydrophobic interaction chromatography;

   Sammanfattning : In recent years shortages of donated blood have become a growing problem for health care systems world-wide. Blood substitutes based on human haemoglobin (Hb) could potentially ameliorate this situation. However, clinical trials have indicated problems due to e.g. LÄS MER

5. 9. Effects of fusion tags on protein partitioning In aqueous two-phase systems and use in primary protein recovery

   Författare :Cynthia Hassinen; KTH; []
   Nyckelord :E. coli; Aqueous two-phase systems; Fusion proteins; Hydrophobic interaction chromatography; Expanded bed adsorption; Beta-galactosidase; Klenow polymerase; Z-domain; Peptide tags;

   Sammanfattning : The two techniques aqueoustwo-phase partitioning and expanded bed adsorption that bothare suitable for primary protein recovery were studied. Most ofthe work was focused on partition in aqueous two-phase systemsand in particular on the possibility to effect the partitionbehaviour by fusion of short peptide tags or protein domains tothe target protein. LÄS MER

7. 10. Improving selectivity in protein extraction by subtle interactions and environmental changes

   Författare :Sheryl Fernandes; Bioteknik; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Difference Spectroscopy; Circular dichroism; Biotechnology; Bioteknik; Chemical technology and engineering; Kemiteknik och kemisk teknologi; Fluorescence spectroscopy; Metal ions; Cibacron blue; Polyvinylpyrrolidone; Polyethylene glycol; Triton X-114; His 6 tag; Cold-active ß-lactamase; Cold-active ß-galactosidase; Lactate dehydrogenase; Cutinase; Affinity Extraction; Conformational change; Partial Denaturation; Molecular interaction; Aqueous two-phase systems; Detergent foam;

   Sammanfattning : The challenges for efficient bioprocesses lie in developing and adapting further current technologies or in creating innovative separation technologies. The thesis is based on the studies on protein recovery from crude feedstocks by extraction in aqueous two-phase systems and detergent foam, respectively. LÄS MER