Sökning: "Z-domain"

Visar resultat 1 - 5 av 23 avhandlingar innehållade ordet Z-domain.

  1. 1. Development of a covalent site-specific antibody labeling strategy by the use of photoactivable Z domains

    Författare :Anna Konrad; Sophia Hober; Hjalmar Brismar; Patrik Samuelsson; KTH; []
    Nyckelord :antibody labeling; site-specific; covalent; SPPS; Z domain; BPA; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

    Sammanfattning : The joining of two molecular functions or the strategy of adding functions to proteins has been tremendously important for the development of proteins as tools in research and clinic. Depending on the intended application, there are a wide variety of functions that can be added to a proteins. LÄS MER

  3. 2. Protein engineering to explore and improve affinity ligands

    Författare :Martin Linhult; KTH; []
    Nyckelord :binding; affinity; human serum albumin HSA ; albumin-binding domain ABD ; affinity chromatography; deamidation; protein A; stabilization; Z-domain; capacity; protein G; cleaning-in-place CIP ; protein engineering; C2 receptor;

    Sammanfattning : In order to produce predictable and robust systems forprotein purification and detection, well characterized, small,folded domains descending from bacterial receptors have beenused. These bacterial receptors, staphylococcal protein A (SPA)and streptococcal protein G (SPG), possess high affinity to IgGand / or HSA. LÄS MER

  4. 3. Effects of fusion tags on protein partitioning In aqueous two-phase systems and use in primary protein recovery

    Författare :Cynthia Hassinen; KTH; []
    Nyckelord :E. coli; Aqueous two-phase systems; Fusion proteins; Hydrophobic interaction chromatography; Expanded bed adsorption; Beta-galactosidase; Klenow polymerase; Z-domain; Peptide tags;

    Sammanfattning : The two techniques aqueoustwo-phase partitioning and expanded bed adsorption that bothare suitable for primary protein recovery were studied. Most ofthe work was focused on partition in aqueous two-phase systemsand in particular on the possibility to effect the partitionbehaviour by fusion of short peptide tags or protein domains tothe target protein. LÄS MER

  5. 4. Molecular principles of protein stability and protein-protein interactions

    Författare :Christofer Lendel; Per-Åke Nygren; Christopher M. Dobson; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; affibody; binding thermodynamics; induced fit; molten globule; NMR spectroscopy; protein engineering; protein-protein interactions; protein stability; protein structure.; Structural biochemistry; Strukturbiokemi;

    Sammanfattning : Proteins with highly specific binding properties constitute the basis for many important applications in biotechnology and medicine. Immunoglobulins have so far been the obvious choice but recent advances in protein engineering have provided several novel constructs that indeed challenge antibodies. LÄS MER

  7. 5. Affibodies

    Författare :Karin Nord; KTH; []
    Nyckelord :;

    Sammanfattning : The introduction of recombinant DNA technology-based methodsfor the overproduction of either wild type or engineeredproteins in various host cells has had an enormous impact onthe availability of proteins for various therapeutic,diagnostic and research applications, which also has creatednew demands on efficient production and purification methods toprovide the desired material. Affinity chromatography hasproven to be an outstanding technique to obtain a single-stepincrease in purity and concentration. LÄS MER