Sökning: "Partial Denaturation"

Visar resultat 1 - 5 av 10 avhandlingar innehållade orden Partial Denaturation.

  1. 1. Improving selectivity in protein extraction by subtle interactions and environmental changes

    Författare :Sheryl Fernandes; Bioteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Difference Spectroscopy; Circular dichroism; Biotechnology; Bioteknik; Chemical technology and engineering; Kemiteknik och kemisk teknologi; Fluorescence spectroscopy; Metal ions; Cibacron blue; Polyvinylpyrrolidone; Polyethylene glycol; Triton X-114; His 6 tag; Cold-active ß-lactamase; Cold-active ß-galactosidase; Lactate dehydrogenase; Cutinase; Affinity Extraction; Conformational change; Partial Denaturation; Molecular interaction; Aqueous two-phase systems; Detergent foam;

    Sammanfattning : The challenges for efficient bioprocesses lie in developing and adapting further current technologies or in creating innovative separation technologies. The thesis is based on the studies on protein recovery from crude feedstocks by extraction in aqueous two-phase systems and detergent foam, respectively. LÄS MER

  3. 2. Biophysical Studies of Protein Folding, Aggregation and Interactions with Molecular Chaperones

    Författare :Per Hammarström; Linköpings universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

    Sammanfattning : The information for a protein to fold correctly into a well defined three-dimensional functional structure is embedded in the primary sequence. Much effort has been devoted to characterize the nature of the unfolded state, since this state represents the starting point of the folding reaction. LÄS MER

  4. 3. The Interaction Between the Chaperonin GroEL and Human Carbonic Anhydrase II : Protein Folding and Stability Studies

    Författare :Malin Persson; Linköpings universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; carbonic anhydrase; protein folding; protein stability; chaperone; GroEL; folding mechanism; site-directed spinlabeling; electron paramagnetic resonance EPR ;

    Sammanfattning : The presence of GroEL/ES during the refolding of human carbonic anhydrase II was found to increase the yield of active enzyme from approx. 70 to approx. 100%. This chaperone action on the enzyme could be obtained by adding GroEL alone, and the time course in that case was only moderately slower than the spontaneous process. LÄS MER

  5. 4. Protein Complexity via Non-Native States: Binding, Stability, and Structural Studies of Calbindin D9k and HAMLET (Human alpha-lactalbumin Made LEthal to Tumor cells)

    Författare :Jonas Fast; Biofysikalisk kemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; apoptosis; oleic acid; HAMLET; kinetic trap alpha-lactalbumin; calbindin; calcium; Metabolism; Biokemi; metabolism;

    Sammanfattning : Using optical spectroscopy, nuclear magnetic resonance (NMR), and differential scanning Calorimetry (DSC), I have studied two different calcium binding proteins that can form kinetically trapped altered states. Calcium is very important in numerous biological processes such as blood coagulation, signal transduction, muscle contraction and bone formation. LÄS MER

  7. 5. Exploring Protein Functions by Molecular Modelling

    Författare :Guido Todde; Sven Hovmöller; Valerie Daggett; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; fysikalisk kemi; Physical Chemistry;

    Sammanfattning : Proteins are one of the most important families of biological macromolecules. Proteins can assume many different structures. This makes them perfect to serve a wide range of functions in all organisms. In the last decades, molecular modeling has become an important and powerful tool in the investigation of biological systems. LÄS MER