Sökning: "His-tag"

Visar resultat 1 - 5 av 6 avhandlingar innehållade ordet His-tag.

  1. 1. The Membrane-Spanning Domain of Complex I Investigated with Fusion Protein Techniques

    Författare :Maria Trane; Lunds universitet.; Lund University.; [2010]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NuoN; NuoM; NuoL; covalent bound heme; Bacillus subtilis; Escherichia coli; membrane protein; His-tag; Cytochrome c; NADH:quinone oxidureductase; fusion protein;

    Sammanfattning : Popular Abstract in Swedish När vi äter kolhydrater, fett, protein och andra näringsämnen sker kemiska reaktioner i kroppen som kan liknas vid dom som sker när man eldar en brasa. Vid förbränning reagerar kolhydrater med syre och bildar koldioxid och vatten. LÄS MER

  2. 2. Ribosomal Stalk Protein L12 Structure, Function and Application

    Detta är en avhandling från Uppsala : Acta Universitatis Upsaliensis

    Författare :Chandra Sekhar Mandava; Uppsala universitet.; [2011]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Ribosome; protein synthesis; L12 dimer; initiation; elongation; G factors; and His-tag; NATURAL SCIENCES Biology Cell and molecular biology Molecular biology; NATURVETENSKAP Biologi Cell- och molekylärbiologi Molekylärbiologi; Biology with specialization in Molecular Biology; Biologi med inriktning mot molekylärbiologi;

    Sammanfattning : Ribosomal stalk proteins are known to play important role in protein synthesis. The ‘stalk’, an extended structure on the large subunit of the ribosome is composed mainly of two to three dimers of L12 and one L10 protein, which forms the base of the stalk. In E. LÄS MER

  3. 3. Protein production, characterization and structure determination in structural genomics

    Detta är en avhandling från Bioteknologi

    Författare :Esmeralda A. Woestenenk; KTH.; [2004]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biology; protein production; protein solubility; fusion tags; nuclear magnetic resonance; structure determination; Biologi; NATURAL SCIENCES Biology; NATURVETENSKAP Biologi;

    Sammanfattning : This thesis covers the process from expression of a heterologous gene in Escherichia coli to structure determination of a protein by nuclear magnetic resonance (NMR) spectroscopy.The first part concerns structural genomics-related parallel screening studies on the effect of fusion tags (in particular the His tag) on protein solubility and the use of fusion tags in fast, parallel purification protocols intended for initial biophysical characterization of human proteins produced in E. LÄS MER

  4. 4. Structural Studies of the Human Water Channel Protein Aquaporin 5

    Detta är en avhandling från Bioteknologi

    Författare :Anna Backmark; [2008]
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; aquaporin 5; aquaporins; X-ray crystallography; twinning; structure; trafficking;

    Sammanfattning : Aquaporins are water channel proteins found in the membranes of all living cells. In humans, thirteen aquaporins have been identified to date. Many of them have been implicated in water imbalance disorders. Water transport through the aquaporins is regulated at several levels. LÄS MER

  5. 5. Glycoside hydrolases from extremophiles isolated in Iceland. alpha-L-Rhamnosidases and pectin degrading enzymes

    Detta är en avhandling från Department of Biotechnology, Lund University

    Författare :Hakon Birgisson; Lunds universitet.; Lund University.; [2004]
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; immobilization; Biotechnology; Bioteknik; crystallization; sequencing; cloning; screening; thermophile; psychrotroph; psychrophile; alpha-L-arabinofuranosidase; alpha-L-rhamnosidase; thermostable; cold active; polygalacturonase;

    Sammanfattning : Extreme environments (high and low temperature, high and low pH, etc.) are inhabited by a vast and diverse flora of microorganisms, which are adapted to these environments (extremophiles). LÄS MER