Sökning: "Electron capture dissociation ECD"

Visar resultat 1 - 5 av 7 avhandlingar innehållade orden Electron capture dissociation ECD.

  1. 1. Characterization of Polypeptides by Tandem Mass Spectrometry Using Complementary Fragmentation Techniques

    Författare :Michael Lund Nielsen; Roman A. Zubarev; Per Andrén; Ole Nörregaard Jensen; Uppsala universitet; []
    Nyckelord :Analytical chemistry; Mass Spectrometry; Electron capture dissociation ECD ; Collision-activated dissociation CAD ; Proteomics; Post-translational modifications; De Novo sequencing; Bioinformatics; Analytisk kemi;

    Sammanfattning : In the growing field of proteomics identification of proteins by tandem mass spectrometry (MS/MS) is performed by matching experimental mass spectra against calculated spectra of all possible peptides in a protein database. One problem with this approach is the false-positive identifications. LÄS MER

  3. 2. Secondary and Higher Order Structural Characterization of Peptides and Proteins by Mass Spectrometry

    Författare :Christopher Adams; Roman Zubarev; Göran Possnert; Carlito Lebrilla; Uppsala universitet; []
    Nyckelord :Engineering physics; Protein Structure; Mass Spectrometry; Electron Capture Dissociation; Tandem Mass Spectrometry; Teknisk fysik;

    Sammanfattning : The work in this thesis has demonstrated the advantages and limitations of using MS based technologies in protein and peptide structural studies. Tandem MS, specifically electron capture dissociation (ECD) have shown the ability to provide structural insights in molecules containing the slightest of all modifications (D-AA substitution). LÄS MER

  4. 3. Analysis of Complex Biological Samples using Liquid Chromatography-Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

    Författare :Margareta Ramström; Jonas Bergquist; Per Håkansson; Karin Markides; Carol Robinson; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Analytical chemistry; mass spectrometry; liquid chromatography; protein; proteomics; peptide; Fourier transform ion cyclotron resonance mass spectrometry; cerebrospinal fluid; amyotrophic lateral sclerosis; electrospray ionization; electron capture dissociation; Analytisk kemi; Analytical chemistry; Analytisk kemi;

    Sammanfattning : Studies of protein and peptide expression are vital in order to understand complex biological systems. As demonstrated in this thesis, on-line packed capillary liquid chromatography-Fourier transform ion cyclotron resonance mass spectrometry (LC-FTICR MS) is a useful analytical tool for such studies. LÄS MER

  5. 4. Strategies to explore the membrane proteome of a cell

    Författare :Gianluca Maddalo; Leopold L. Ilag; Peter James; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Membrane proteins; Proteomics; Escherichia coli; Enterococcus faecalis; Synechocystis; Mass Spectrometry; BN SDS-PAGE; FT-ICR-MS; Myelin P2 protein; Analytical chemistry; Analytisk kemi; Analytical Chemistry; analytisk kemi;

    Sammanfattning : The cell envelope plays key roles in numerous processes such as maintaining cellular integrity, communication with other cells, signal transduction, maintenance of cellular homeostasis, and regulation of the traffic of molecules between the cell and the extracellular milieu. Essential membrane components in many of these processes are proteins. LÄS MER

  7. 5. New Proteomics Methods and Fundamental Aspects of Peptide Fragmentation

    Författare :Mikhail Savitski; Roman Zubarev; Per Andren; David Fenyö; Uppsala universitet; []
    Nyckelord :Bioinformatics; Proteomics; Peptide fragmentation; Bioinformatik;

    Sammanfattning : The combination of collision-activated dissociation, (CAD) and electron capture dissociation, (ECD) yielded a 125% increase in protein identification. The S-score was developed for measuring the information content in MS/MS spectra. This measure made it possible to single out good quality spectra that were not identified by a search engine. LÄS MER