Sökning: "Roman Zubarev"

Hittade 5 avhandlingar innehållade orden Roman Zubarev.

  1. 1. Mass spectrometry of biopolymers based on ion-induced desorption

    Författare :Roman Alexandrovich Zubarev; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Radiation sciences; Strålningsvetenskap; Radiation biology; Strålningsbiologi; Ion Physics; jonfysik;

    Sammanfattning : Various aspects of biopolymer molecular characterization by their molecular masses havebeen studied, with emphasis on ion-induced desorption and time-of-flight mass Spectrometry(TOF MS). Limitations of the average-isotopic mass concept, as well as the utility ofaccurate monoisotopic mass measurements for deriving amino-acid and elementalcompositions, are discussed. LÄS MER

  3. 2. Secondary and Higher Order Structural Characterization of Peptides and Proteins by Mass Spectrometry

    Författare :Christopher Adams; Roman Zubarev; Göran Possnert; Carlito Lebrilla; Uppsala universitet; []
    Nyckelord :Engineering physics; Protein Structure; Mass Spectrometry; Electron Capture Dissociation; Tandem Mass Spectrometry; Teknisk fysik;

    Sammanfattning : The work in this thesis has demonstrated the advantages and limitations of using MS based technologies in protein and peptide structural studies. Tandem MS, specifically electron capture dissociation (ECD) have shown the ability to provide structural insights in molecules containing the slightest of all modifications (D-AA substitution). LÄS MER

  4. 3. Multiplexed absolute quantification of blood plasma proteins using targeted proteomics

    Författare :David Kotol; Mathias Uhlén; Edfors Fredrik; Roman Zubarev; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; mass spectrometry; targeted proteomics; stable isotope standards; recombinant protein standards; absolute quantification; blood plasma; multiplex analysis; plasma profiling; Biotechnology; Bioteknologi;

    Sammanfattning : Proteins are the molecular building blocks of all living organisms. They are the functioning actors in metabolism and communication, and they give specific architecture and purpose to all tissues. Their abundance is dynamic and can be interpreted and associated to different physiological states or diseases. LÄS MER

  5. 4. Characterization of Polypeptides by Tandem Mass Spectrometry Using Complementary Fragmentation Techniques

    Författare :Michael Lund Nielsen; Roman A. Zubarev; Per Andrén; Ole Nörregaard Jensen; Uppsala universitet; []
    Nyckelord :Analytical chemistry; Mass Spectrometry; Electron capture dissociation ECD ; Collision-activated dissociation CAD ; Proteomics; Post-translational modifications; De Novo sequencing; Bioinformatics; Analytisk kemi;

    Sammanfattning : In the growing field of proteomics identification of proteins by tandem mass spectrometry (MS/MS) is performed by matching experimental mass spectra against calculated spectra of all possible peptides in a protein database. One problem with this approach is the false-positive identifications. LÄS MER

  7. 5. New Proteomics Methods and Fundamental Aspects of Peptide Fragmentation

    Författare :Mikhail Savitski; Roman Zubarev; Per Andren; David Fenyö; Uppsala universitet; []
    Nyckelord :Bioinformatics; Proteomics; Peptide fragmentation; Bioinformatik;

    Sammanfattning : The combination of collision-activated dissociation, (CAD) and electron capture dissociation, (ECD) yielded a 125% increase in protein identification. The S-score was developed for measuring the information content in MS/MS spectra. This measure made it possible to single out good quality spectra that were not identified by a search engine. LÄS MER