Sökning: "Mass Spectrometry for Proteomics"

Visar resultat 1 - 5 av 162 avhandlingar innehållade orden Mass Spectrometry for Proteomics.

1. 1. Mass Spectrometry-based Neuroproteomics : Deciphering the Human Brain Proteome

   Författare :Sravani Musunuri; Jonas Bergquist; Ganna Shevchenko; Jean-Charles Sanchez; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Brain; Proteomics; Mass spectrometry; Alzheimer s disease; Traumatic brain injury; Membrane proteins; Sample preparation; Kemi med inriktning mot analytisk kemi; Chemistry with specialization in Analytical Chemistry;

   Sammanfattning : Mammalian brain is challenging to study due to its heterogeneity and complexity. However, recent advances in molecular imaging, genomics and proteomics have contributed significantly to achieve insights into molecular basis of brain function and pathogenesis of neurological disorders. LÄS MER

3. 2. Development of Methods for Protein and Peptide Analysis Applied in Neuroscience Utilizing Mass Spectrometry

   Författare :Johan Pierson; Per Andrén; Ulf Bondesson; György Marko-Varga; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Analytical chemistry; Mass spectrometry; Parkinson’s disease; Metabolism; Profiling Mass Spectrometry; Proteomics; Analytisk kemi; Analytical chemistry; Analytisk kemi;

   Sammanfattning : This thesis describes the utilization of the matrix-assisted laser desorption ionization mass spectrometry (MALDI MS) and electrospray ionization (ESI) MS techniques for analysis of complex brain tissue samples. Direct molecular profiling of biological samples using MALDI MS is a powerful tool for identifying phenotypic markers. LÄS MER

4. 3. Advances for Biomarker Discovery in Neuroproteomics using Mass Spectrometry : From Method Development to Clinical Application

   Författare :Marcus O.D. Sjödin; Magnus Wetterhall; Jonas Bergquist; Dominic M. Desiderio; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Mass Spectrometry; Biomarker; Proteomics; Central Nervous System; Cerebrospinal Fluid; Traumatic Brain Injury; Cloud-Point Extraction; Neuroproteomics; Relative Quantification; Spinal Cord Stimulation; Neuropathic Pain; Analytical Chemistry; Analytisk kemi;

   Sammanfattning : Proteins offer a prominent group of compounds which may be ubiquitously affected in disease and used as biomarkers for early diagnosis, assessing treatment or drug development. Clinical proteomics aim to screen for protein biomarkers by a comprehensive analysis of all proteins expressed in a biological matrix during a certain pathology. LÄS MER

5. 4. High-throughput protein analysis using mass spectrometry-based methods

   Författare :Tove Boström; Sophia Hober; Thomas Laurell; Ole N. Jensen; KTH; []
   Nyckelord :Proteomics; mass spectrometry; affinity proteomics; immunoenrichment; immunoprecipitation; IMAC; screening; protein production; protein purification; ISET; quantification; SILAC; stable isotope standard; antibody validation; Bioteknologi; Biotechnology;

   Sammanfattning : In the field of proteomics, proteins are analyzed and quantified in high numbers. Protein analysis is of great importance and can for example generate information regarding protein function and involvement in disease. Different strategies for protein analysis and quan- tification have emerged, suitable for different applications. LÄS MER

7. 5. Strategies for Miniaturized Biomarker Detection

   Författare :Belinda Adler; Avdelningen för Biomedicinsk teknik; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; biomarker; miniturization; lab-on-a-chip; proteomics; PSA; microarray; mass spectrometry; MALDI MS; SPE; sample preparation; immunoaffinity; antibody; aptamer; mass tag; ISET.;

   Sammanfattning : The aim of this thesis is development and application of different miniaturized strategies for detection of biomarkers. The biomarker PSA (prostate specific antigen), which is prostate cancer specific, has been the main focus of the thesis. LÄS MER