Sökning: "electron capture dissociation"

Visar resultat 1 - 5 av 16 avhandlingar innehållade orden electron capture dissociation.

1. 1. High Rate Electron Capture Dissociation Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

   Författare :Youri Tsybin; Per Håkansson; Ron M.A. Heeren; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Physics; mass spectrometry; Fourier transform ion cyclotron resonance; peptides and proteins; ion-electron interaction; electron capture dissociation; infrared multiphoton dissociation; liquid chromatography; capillary electrophoresis; Fysik; Physics; Fysik;

   Sammanfattning : Advances in science and technology during the past decade have greatly enhanced the level of the structural investigation of macromolecules – peptides and proteins. Biological mass spectrometry has become one of the most precise and sensitive techniques in peptide and protein analysis. LÄS MER

3. 2. Ultrafast Photoionization Dynamics Studied with Coincidence Momentum Imaging Spectrometers

   Författare :Yu-Chen Cheng; Atomfysik; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Photoionization; High-order Harmonic Generation; Extreme Ultraviolet; Free-Electron Laser; Molecular Dissociation; Charge Transfer; Coincidence Measurement; Momentum Imaging; Electron Wave Packet; Fysicumarkivet A:2019:Cheng;

   Sammanfattning : The time scale of the dynamics in atoms and molecules varies from attoseconds (10-18) to picoseconds (10-12) depending on the size of the particles. To study such dynamics, ultrafast light pulses are needed to trigger and capture the reaction. One of the most direct methods consists in ionizing the system and observing the following response. LÄS MER

4. 3. Secondary and Higher Order Structural Characterization of Peptides and Proteins by Mass Spectrometry

   Författare :Christopher Adams; Roman Zubarev; Göran Possnert; Carlito Lebrilla; Uppsala universitet; []
   Nyckelord :Engineering physics; Protein Structure; Mass Spectrometry; Electron Capture Dissociation; Tandem Mass Spectrometry; Teknisk fysik;

   Sammanfattning : The work in this thesis has demonstrated the advantages and limitations of using MS based technologies in protein and peptide structural studies. Tandem MS, specifically electron capture dissociation (ECD) have shown the ability to provide structural insights in molecules containing the slightest of all modifications (D-AA substitution). LÄS MER

5. 4. Characterization of Polypeptides by Tandem Mass Spectrometry Using Complementary Fragmentation Techniques

   Författare :Michael Lund Nielsen; Roman A. Zubarev; Per Andrén; Ole Nörregaard Jensen; Uppsala universitet; []
   Nyckelord :Analytical chemistry; Mass Spectrometry; Electron capture dissociation ECD ; Collision-activated dissociation CAD ; Proteomics; Post-translational modifications; De Novo sequencing; Bioinformatics; Analytisk kemi;

   Sammanfattning : In the growing field of proteomics identification of proteins by tandem mass spectrometry (MS/MS) is performed by matching experimental mass spectra against calculated spectra of all possible peptides in a protein database. One problem with this approach is the false-positive identifications. LÄS MER

7. 5. Identification and Characterization of Peptides and Proteins using Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

   Författare :Magnus Palmblad; Ole Nørregaard Jensen; Uppsala universitet; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Materials science; Peptide; protein; peptide mass fingerprinting; identification; sequencing; protein structure; non-covalent interaction; modification; electrospray ionization; liquid chromatography; capillary electrophoresis; electron capture dissociation; Fourier transform ion cyclotron resonance mass spectrometry; cerebrospinal fluid; amyloid β-peptide; Alzheimer’s disease.; Materialvetenskap; Materials science; Teknisk materialvetenskap; Molecular Biotechnology; Molekylär bioteknik;

   Sammanfattning : Mass spectrometry has in recent years been established as the standard method for protein identification and characterization in proteomics with excellent intrinsic sensitivity and specificity. Fourier transform ion cyclotron resonance is the mass spectrometric technique that provides the highest resolving power and mass accuracy, increasing the amount of information that can be obtained from complex samples. LÄS MER