Sökning: "C. coli"

Visar resultat 11 - 15 av 186 avhandlingar innehållade orden C. coli.

  1. 11. Cystatin C functions in vitro and in vivo. Studies on target enzyme inhibition by cystatin C variants and cystatin C deficient mice

    Författare :Katarina Håkansson; Avdelningen för klinisk kemi och farmakologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; amyloidosis; E.coli expression; Cysteine proteases; protease inhibitors; Clinical chemistry; Klinisk kemi;

    Sammanfattning : Cystatin C variants with Arg8, Leu9 and/or Val10 replaced by Gly residues were produced by site-directed mutagenesis and E. coli expression. LÄS MER

  3. 12. The Membrane-Spanning Domain of Complex I Investigated with Fusion Protein Techniques

    Författare :Maria Trane; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NuoN; NuoM; NuoL; covalent bound heme; Bacillus subtilis; Escherichia coli; membrane protein; His-tag; Cytochrome c; NADH:quinone oxidureductase; fusion protein;

    Sammanfattning : NADH:quinone oxidoreductase, or complex I, is a large, complex and poorly understood bioenergetic enzyme in the respiratory chain of living organisms. The enzyme has a conserved core structure, comprising fourteen protein subunits: Seven subunits protrude from the membrane and contain a flavin prosthetic group and eight iron-sulfur clusters that guide electrons from the oxidation of NADH towards the quinone binding site(s). LÄS MER

  4. 13. Nucleotide Binding to Trimeric dUTPase: Studies on Recombinant Enzymes using Kinetic and Spectroscopic Methods

    Författare :Johan Nord; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; C-terminus; dUTPase; EIAV; Escherichia coli; flexibility; Homo sapiens; kinetics; NMR; nucleotide metabolism; rate constants of lig-and binding; production of recombinant enzyme; dUTP; specificity; Biochemistry; Metabolism; Biokemi;

    Sammanfattning : Recombinant dUTPase from the bacterium Escherichia coli, the retrovirus equine infectious anemia virus (EIAV) and Homo sapiens have been made available for physico-chemical characterisation. The protocols developed have also proven to be suitable for production of enzymes modified by isotope labelling or other methods. LÄS MER

  5. 14. Translational accuracy, ribozyme efficiency and NAD metabolism in Escherichia coli

    Författare :Martin Stancek; Leif Isaksson; Lars Hederstedt; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; translational accuracy; nicotinate mononucleotide adenylyltransferase; Microbiology; Mikrobiologi;

    Sammanfattning : Interactions between the translational machinery and mRNA in Escherichia coli have been studied. In the first section nonsense codon readthrough and changed translational reading frame were measured in different growth phases. LÄS MER

  7. 15. Enhancing membrane and secretory protein production yields in Escherichia coli

    Författare :Zhe Zhang; Jan-Willem de Gier; Claes von Wachenfeldt; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; recombinant protein production; Escherichia coli; membrane protein; secretory protein; strain engineering; Biochemistry; biokemi;

    Sammanfattning : Proteins fulfill essential functions in living cells. To produce sufficient amounts of a protein is essential to study the structure and function of a protein, or to use it for medical purposes. Escherichia coli is a Gram-negative bacterium that is widely used for recombinant protein production. LÄS MER