Sökning: "nucleotide metabolism"

Visar resultat 1 - 5 av 100 avhandlingar innehållade orden nucleotide metabolism.

  1. 1. Cyclic Nucleotide Phosphodiesterase 3B: Regulation in rat adipocytes and 3T3-L1 cells

    Författare :Tova Landström; Institutionen för experimentell medicinsk vetenskap; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; 3T3-L1 TNF-alpha; adipocyte; insulin; p70S6 kinase; mitogen-activated protein kinase; protein kinase B; phosphatidylinositol-3 kinase; phosphodiesterase 3B; cAMP; lipolysis; phosphorylation; signal transduction; Biochemistry; Metabolism; Biokemi; metabolism;

    Sammanfattning : Insulin stimulation of rat adipocytes results in phosphorylation and activation of the cyclic nucleotide phosphodiesterase 3B (PDE3B), a key enzyme in the antilipolytic signalling pathway of this hormone. In this thesis, the site phosphorylated in PDE3B upon stimulation of rat adipocytes with insulin and/or the beta-adrenergic agonist, isoproterenol is identified. LÄS MER

  3. 2. Nucleotide Binding to Trimeric dUTPase: Studies on Recombinant Enzymes using Kinetic and Spectroscopic Methods

    Författare :Johan Nord; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; C-terminus; dUTPase; EIAV; Escherichia coli; flexibility; Homo sapiens; kinetics; NMR; nucleotide metabolism; rate constants of lig-and binding; production of recombinant enzyme; dUTP; specificity; Biochemistry; Metabolism; Biokemi;

    Sammanfattning : Recombinant dUTPase from the bacterium Escherichia coli, the retrovirus equine infectious anemia virus (EIAV) and Homo sapiens have been made available for physico-chemical characterisation. The protocols developed have also proven to be suitable for production of enzymes modified by isotope labelling or other methods. LÄS MER

  4. 3. A beta-phosphoglucomutase in carbohydrate metabolism of Lactococcus lactis

    Författare :Ny Qian; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; mykologi; virologi; bakteriologi; Mikrobiologi; mycology; gene regulation; beta-phosphoglucomutase; maltose metabolism; lactate; metabolic engineering; alpha-phosphoglucomutase; Lactococcus lactis; Microbiology; virology; bacteriology; Biochemistry; Metabolism; Biokemi;

    Sammanfattning : Phosphoglucomutase (PGM) catalyzes the reversible conversion of glucose 1-phosphate to glucose 6-phosphate. Two distinct forms of PGM have been purifed and charactierized in Lactococcus lactis growing on maltose: one specific for the alpha-isomer, and the other specific for the beta-isomer of glucose 1-phosphate. LÄS MER

  5. 4. On Monomeric and Trimeric dUTPases. Recombinant Expression, Purification, Conformational Properties and Catalytic Characteristics

    Författare :Rebecca Persson; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Metabolism; dUTP; dUTPase; nucleotide metabolism; retrovirus; crystallography; herpesvirus; Biokemi; Biochemistry;

    Sammanfattning : Nucleotide metabolism and replication of nucleic acids are processes of fundamental importance. A crucial enzyme in nucleotide metabolism is dUTPase (E.C. 3. LÄS MER

  7. 5. Nucleotide-Dependent Processes in the Thylakoid Lumen of Plant Chloroplasts

    Författare :Björn Lundin; Cornelia Spetea Wiklund; Zach Adam; Linköpings universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; plant; chloroplast; thylakoid; nucleotide; PSII; PsbO; Biology; Biologi;

    Sammanfattning : Plants, algae and photosynthetic bacteria are able to harvest the sunlight and use its energy to transform water and carbon dioxide to carbohydrate molecules and oxygen, both important to sustain life on Earth. This process is called photosynthesis and is the route by which almost all energy enters the biosphere. LÄS MER