Sökning: "two-dimensional liquid chromatography"

Visar resultat 6 - 10 av 22 avhandlingar innehållade orden two-dimensional liquid chromatography.

  1. 6. Analysis of drugs and proteins in biological matrices, using different types of sample preparations and mass spectrometry

    Författare :Aljona Saleh; Ingrid Granelli; Jonas Bergquist; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Bioanalysis; drugs; proteins; liquid chromatography; two-dimensional liquid chromatography; mass spectrometry; Analytical Chemistry; analytisk kemi;

    Sammanfattning : This thesis describes bioanalysis of small and large molecules in biological matrices and includes screening of illegal drugs in urine with high resolution mass spectrometer, bioanalysis of MTH1 inhibitors in mice plasma and quantification of proteins in plasma and cell lysates.Screening of illegal drugs was based on high resolving power mass spectrometer (QTOF) and the results were compared to immunoassays. LÄS MER

  3. 7. Interfacing HPLC with MALDI- and ESI-MS for Automated High Sensitivity Analysis of Proteins and Peptides

    Författare :Tasso Miliotis; Lund Institutionen för kliniska vetenskaper; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; automated; phosphorylation; ZAP-70; peptides; proteins; comprehensive; multidimensional; two-dimensional; piezoelectric; microdispenser; flow-through; ESI-MS MS; Capillary HPLC; MALDI-TOF MS; Analytical chemistry; Analytisk kemi;

    Sammanfattning : The research described in this thesis has an interdisciplinary approach dealing with chemical, biological, and technical issues. One objective was to interface miniaturized liquid chromatography (LC) to mass spectrometry, i.e. MALDI-TOF MS and ESI-MS, in an unattended mode for high sensitivity determination of proteins and peptides. LÄS MER

  4. 8. Neuropeptidomics – Expanding Proteomics Downwards

    Författare :Marcus Svensson; Per Andrén; Per Svenningsson; Jonathan Sweedler; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Pharmaceutical pharmacology; proteomics; peptidomics; mass spectrometry; neuropeptide; chromatography; Farmaceutisk farmakologi; PHARMACY; FARMACI;

    Sammanfattning : Biological function is mainly carried out by a dynamic population of proteins which may be used as markers for disease diagnosis, prognosis, and as a guide for effective treatment. In analogy to genomics, the study of proteins is called proteomics and it is generally performed by two-dimensional gel electrophoresis and mass spectrometric methods. LÄS MER

  5. 9. Development and application of a proteomic approach to the assessment of pollution in the marine environment

    Författare :Itxaso Apraiz Larrucea; Susana Cristobal; Joe de Pierre; David Sheehan; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proteomics; mussels; peroxisomes; two-dimensional electrophoresis 2-DE ; difference gel electrophoresis DIGE ; bisphenol A BPA ; diallyl phthalate DAP ; 2; 2′; 4; 4′-tetrabromodiphenyl ether PBDE-47 ; fuel oil; Prestige oil spill; Environmental toxicology; Miljötoxikologi; Toxicology; Toxikologi; Biochemistry; biokemi;

    Sammanfattning : Today, assessment of the health of coastal waters is recognized as being important for both the conservation of nature and well-being of humans. Anthropogenic pollution has been the focus of extensive research for some time and a variety of programs for the monitoring and assessment of environmental pollution have been developed. LÄS MER

  7. 10. PLE with integrated clean up followed by alternative detection steps for cost-effective analysis of dixons and dioxin-like compounds

    Författare :Erik Spinnel; Peter Haglund; Patrik Andersson; Tuulia Hyötyläinen; Umeå universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Pressurized liquid extraction PLE ; PLE-C; modular PLE; ELISA; dioxin; dioxin-like compounds; GCxGC; analytical methods; Environmental chemistry; Miljökemi;

    Sammanfattning : Polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs) are two structurally related groups of chemicals, generally referred to as `dioxins´. These are of great concern due to their high toxicity and global spread. LÄS MER