Sökning: "protein throughput"

Visar resultat 26 - 30 av 259 avhandlingar innehållade orden protein throughput.

  1. 26. Mammalian protein expression and characterization tools for next generation biologics

    Författare :Niklas Thalén; Johan Rockberg; Kerstin Otte; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; CHO; Cell line engineering; Protein engineering; GFP; Cell display; Biotechnology; Bioteknologi;

    Sammanfattning : Protein therapeutics are increasingly important for modern medicine. Novel recombinant proteins developed today can bind towards their target with high specificity and with low adverse effect. This has enabled the treatment of diseases that for a few years ago were deemed uncurable. LÄS MER

  3. 27. Selection of antigens for antibody-based proteomics

    Författare :Lisa Berglund; Mathias Uhlén; Sören Brunak; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; epitope; antigen; antibody; affinity; protein; proteome; proteomics; bioinformatics; prediction; primer design; sequence similarity; Bioengineering; Bioteknik;

    Sammanfattning : The human genome is predicted to contain ~20,500 protein-coding genes. The encoded proteins are the key players in the body, but the functions and localizations of most proteins are still unknown. LÄS MER

  4. 28. Design of antibody microarrays for global profiling of membrane proteins and soluble proteins

    Författare :Linda Dexlin Mellby; Institutionen för immunteknologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; scFv; membrane proteins; proteomics; protein chip; recombinant antibodies; Antibody microarray; protein profiling; membrane proteomics;

    Sammanfattning : Antibody-based microarrays have emerged as an established proteomic technology allowing multiplexed and sensitive profiling of complex proteomes, in a high-throughput and miniaturized manner. Recently, numerous applicative efforts have been pursued generating disease-associated protein signatures that now could be further explored for improved disease diagnostics, prognostics and classification. LÄS MER

  5. 29. Characterization and Crystallization of Anchorless Glypican-1

    Författare :Gabriel Svensson Birkedal; Glykobiologigruppen; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; S-nitrosylation; nitric oxide; protein stability; protein conformation; X-ray crystallography; calorimetry; spectroscopy; proteoglycan; glypican; heparan sulfate;

    Sammanfattning : Glypicans are cell-surface heparan sulfate proteoglycans that regulate Wnt, Hedgehog, bone morphogenetic protein, and fibroblast growth factor signaling through their heparan sulfate chains. Recent studies have shown that glypican core proteins also have functional roles in growth factor signaling, but biochemical and structural knowledge regarding the core proteins is limited. LÄS MER

  7. 30. Mass Spectrometry and Affinity Based Methods for Analysis of Proteins and Proteomes

    Författare :Mårten Sundberg; Margareta Ramström; Jonas Bergquist; Christer Wingren; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Mass spectrometry; proteomics; microarray; protein; antibody; antigen; affinity; validation; Kemi med inriktning mot analytisk kemi; Chemistry with specialization in Analytical Chemistry;

    Sammanfattning : Proteomics is a fast growing field and there has been a tremendous increase of knowledge the last two decades. Mass spectrometry is the most used method for analysis of complex protein samples. It can be used both in large scale discovery studies as well as in targeted quantitative studies. LÄS MER