Sökning: "protein conformation"

Visar resultat 1 - 5 av 258 avhandlingar innehållade orden protein conformation.

1. 1. Structure determination and thermodynamic stabilization of an engineered protein-protein complex

   Författare :Elisabet Wahlberg; Per-Åke Nygren; Bengt-Harald Jonsson; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; affibody; protein structure; coupled folding; NMR spectroscopy; protein stability; protein-protein interactions; binding thermodynamics; isothermal titration calorimetry; protein engineering; Structural biochemistry; Strukturbiokemi;

   Sammanfattning : The interaction between two 6 kDa proteins has been investigated. The studied complex of micromolar affinity (Kd) consists of the Z domain derived from staphylococcal protein A and the related protein ZSPA-1, belonging to a group of binding proteins denoted affibody molecules generated via combinatorial engineering of the Z domain. LÄS MER

3. 2. Exploring small heat shock protein chaperones by crosslinking mass spectrometry

   Författare :Wietske Lambert; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; cross-linking; mass spectrometry; protein-protein interactions; Hsp21; BS3; DTSSP; MALDI-TOF TOF; client protein; substrate; aggregation; unfolded protein; chaperone; Small heat shock protein; crosslinking;

   Sammanfattning : Together with other molecular chaperones, small heat shock proteins are key components of the protein quality control system, which is comprised of several hundred proteins and acts to maintain proteome homeostasis in the cell. Small heat shock proteins bind unfolding proteins at an early stage, to prevent these from further unfolding and aggregating. LÄS MER

4. 3. Protein-protein interactions in model systems : design, control of catalytic activity and biosensor applications

   Författare :Johan Rydberg; Lars Baltzer; Joel Schneider; Linköpings universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Chemistry; Protein interactions; design; catalysis; biosensors; hybride materials; nanoparticles; Kemi; Chemistry; Kemi;

   Sammanfattning : This thesis describes the design of polypeptides, unordered in the monomeric state but capable of folding into helix-loop-helix motifs and dimerise to form four-helix bundles. The goal of the design was to encode them with the capacity to form dimers highly selectively and the ability to carry out molecular functions in the folded state but not in the unordered state, and thus to establish a molecular link between recognition and function. LÄS MER

5. 4. Angiopoietin-like protein 4 : an unfolding chaperone regulating lipoprotein lipase activity

   Författare :Valentina Sukonina; Gunilla Olivecrona; Lena Kjellen; Umeå universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Lipoprotein lipase; angiopoietin-like protein 4; protein folding; human adipose tissue; macrophages; peroxisome proliferator-activated receptor delta PPARδ ; Medical microbiology; Medicinsk mikrobiologi;

   Sammanfattning : Lipoprotein lipase (LPL) is the main enzyme hydrolyzing triglyceride-rich lipoproteins in plasma. Proteoglycan-bound LPL on the vascular endothelium represent the functional pool of active enzyme. LPL is regulated in a tissue specific manner according to metabolic demands. LÄS MER

7. 5. Material Properties of Cereal Protein Films

   Författare :Thomas Gillgren; Chalmers tekniska högskola; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; plasticization; avenin; prolamin; kafirin; mechanical properties; water vapour permeability; oxygen permeability; protein; film; glass transition temperature; zein;

   Sammanfattning : Materials that are renewable and biodegradable are demanded due to environmental aspects. Biopolymeric materials, such as materials from prolamins, fulfil both of these requirements. Prolamins are cereal storage proteins. Examples of such are zein, gluten, kafirin and avenin from maize, wheat, sorghum and oats, respectively. LÄS MER