Sökning: "membrane proteomics"

Visar resultat 1 - 5 av 65 avhandlingar innehållade orden membrane proteomics.

  1. 1. Dementia Proteomics

    Författare :Julia Remnestål; Peter Nilsson; Anna Månberg; Pieter Jelle Visser; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Dementia; Proteomics; Alzheimer s disease; Frontotemporal dementia; Antibody array; Suspension bead array; Cerebrospinal fluid; Protein profiling; Biomarker discovery; Bioteknologi; Biotechnology;

    Sammanfattning : The term dementia encompass a number of conditions arising as a consequence of tissue degeneration in the brain. This degeneration is caused by molecular events occurring on a cellular level including inflammation, defective waste disposal and accumulation of insoluble proteins and peptides. LÄS MER

  3. 2. Into the Membrane and Beyond : Improving Membrane Protein Over-Expression in Escherichia coli

    Författare :Mirjam Klepsch; Jan-Willem de Gier; Reinhard Grisshammer; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; membrane protein over-expression; proteomics; T7 RNA polymerase; peptide transport; strain engineering; Biochemistry; Biokemi; Biophysical chemistry; Biofysikalisk kemi; biokemi; Biochemistry;

    Sammanfattning : Membrane proteins fulfil a wide variety of essential functions in the cell and many are (potential) drug targets. Since their natural abundance is usually very low, most membrane proteins have to be over-expressed for functional and structural studies. LÄS MER

  4. 3. Design of antibody microarrays for global profiling of membrane proteins and soluble proteins

    Författare :Linda Dexlin Mellby; Institutionen för immunteknologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; scFv; membrane proteins; proteomics; protein chip; recombinant antibodies; Antibody microarray; protein profiling; membrane proteomics;

    Sammanfattning : Antibody-based microarrays have emerged as an established proteomic technology allowing multiplexed and sensitive profiling of complex proteomes, in a high-throughput and miniaturized manner. Recently, numerous applicative efforts have been pursued generating disease-associated protein signatures that now could be further explored for improved disease diagnostics, prognostics and classification. LÄS MER

  5. 4. Membrane protein proteomics - Novel method for membrane protein identification and quantification

    Författare :Maria Bentz; Institutionen för immunteknologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biokemisk teknik; mebran; steroider; Lipider; membranes; steroids; Lipids; enzymologi; Proteiner; Membrane proteins; Proteomics; Proteinase K; N-terminal labelling; Relative Quantitation; Database search; Cancer; Cytology; oncology; cancerology; Cytologi; onkologi; cancer; enzymology; Proteins; Biochemical technology;

    Sammanfattning : Membrane proteins are fairly refractory to digestion especially by trypsin. Less specific proteases like elastase and pepsin are much more effective. However database searching using non-tryptic peptides is much less effective due to the lack of charge localisation at the N- and C-termini and the absence of sequence specificity. LÄS MER

  7. 5. Membrane Protein Biogenesis in Escherichia coli : A proteomics approach

    Författare :David Vikström; Jan-Willem de Gier; Eitan Bibi; Stockholms universitet; []
    Nyckelord :SRP; YidC; SecE; Membrane protein biogenesis; protein targeting; 2D BN SDS-PAGE; Biochemistry; biokemi;

    Sammanfattning : In the bacterium Escherichia coli all proteins are synthesized in the cytoplasm. However, at least 25% of them then need to be exported to another cellular compartment where they exert their function. E. coli has several different targeting pathways to ensure the correct localization of its proteins. LÄS MER