Sökning: "fluorescent reporter"

Visar resultat 6 - 10 av 47 avhandlingar innehållade orden fluorescent reporter.

  1. 6. Amyloid Beta – Biotherapy Target and Biotechnological Tool

    Författare :Sebastian W. Meister; Stefan Ståhl; John Löfblom; Maarten Merkx; KTH; []
    Nyckelord :Alzheimer’s disease; amyloid beta; blood-brain barrier; green-fluorescent protein; intracellular assays; proteases; protease substrate specificity; protein aggregation; protein engineering.; Bioteknologi; Biotechnology;

    Sammanfattning : Alzheimer’s disease is a progressive neurodegenerative disease characterized primarily by memory impairment and cognitive decline. As of 2020, an estimated 50 million people suffer from Alzheimer’s disease or related dementia and no disease-modifying treatment options are yet approved for clinical practice. LÄS MER

  3. 7. Recognition and Visualization of Nucleic Acids

    Författare :Marcus Wilhelmsson; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; intercalation; cyanine dye; ruthenium; polarized spectroscopy; threading; groove-binding; kinetics; genetic screening; DNA; fluorescent base analogue;

    Sammanfattning : The work presented in this Thesis focuses on the interaction between nucleic acids and small molecules, as well as binding or incorporation of reporter chromophores into nucleic acids. The results could help in studying and understanding processes that are fundamental to describe mechanisms behind a genetic disorder. LÄS MER

  4. 8. Staphylococcus aureus toxins - Influence on food safety and animal health

    Författare :Yusak Budi Susilo; Teknisk mikrobiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Staphylococcus aureus; toxins; food safety; enterotoxin A SEA ; prophage life cycle; GFP reporter strain; flow cytometry; enterotoxin D SED ; accessory gene regulator agr ; bovine mastitis; enterotoxin C SEC ; toxic shock syndrome toxin-1 TSST-1 ;

    Sammanfattning : Staphylococcus aureus (S. aureus) is a notorious opportunistic foodborne pathogen and also a common cause of bovine mastitis. It is known to produce many different virulence factors, including various staphylococcal enterotoxins and toxic shock syndrome toxin-1 (TSST-1). LÄS MER

  5. 9. Small RNAs, Big Consequences : Post-transcriptional Regulation and Adaptive Immunity in Bacteria

    Författare :Mirthe Hoekzema; Gerhart E. H. Wagner; Magnus Lundgren; Kai Papenfort; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; small RNA; sRNA; non-coding RNA; Hfq; gene regulation; post-transcriptional regulation; biofilm; OmrA; OmrB; toxin-antitoxin; TisB; IstR-1; Persisters; CRISPR-Cas; CRISPR-Cas adaptation reporter; Escherichia coli; Biologi med inriktning mot mikrobiologi; Biology with specialization in Microbiology;

    Sammanfattning : It is nowadays widely accepted that non-coding RNAs play important roles in post-transcriptional regulation of genes in all kingdoms of life. In bacteria, the largest group of RNA regulators are the small RNAs (sRNAs). LÄS MER

  7. 10. The ubiquitin-proteasome system during proteotoxic stress

    Författare :Victoria Menéndez Benito; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Ubiquitin; proteasome; green fluorescent protein GFP ; degradation; inhibitor; neurodegeneration; stress;

    Sammanfattning : The dual function of the ubiquitin-proteasome system in protein quality control and as a master regulator of vital cellular processes places the system in a delicate position. This might be particularly relevant under patho-physiological conditions such as endoplasmic reticulum stress, which provoke the accumulation of aberrant proteins. LÄS MER