Sökning: "CRISPR-Cas"

Visar resultat 1 - 5 av 6 avhandlingar innehållade ordet CRISPR-Cas.

  1. 1. The type I-E CRISPR-Cas system : Biology and applications of an adaptive immune system in bacteria

    Författare :Lina Amlinger; Magnus Lundgren; Peter Fineran; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; CRISPR; CRISPR-Cas; virus defense; bacteria; bacteriophage; adaptation; spacer integration; interference; gene silencing; fluorescent reporter; Escherichia coli; Mikrobiologi; Microbiology;

    Sammanfattning : CRISPR-Cas systems are adaptive immune systems in bacteria and archaea, consisting of a clustered regularly interspaced short palindromic repeats (CRISPR) array and CRISPR associated (Cas) proteins. In this work, the type I-E CRISPR-Cas system of Escherichia coli was studied.CRISPR-Cas immunity is divided into three stages. LÄS MER

  3. 2. Small RNAs, Big Consequences : Post-transcriptional Regulation and Adaptive Immunity in Bacteria

    Författare :Mirthe Hoekzema; Gerhart E. H. Wagner; Magnus Lundgren; Kai Papenfort; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; small RNA; sRNA; non-coding RNA; Hfq; gene regulation; post-transcriptional regulation; biofilm; OmrA; OmrB; toxin-antitoxin; TisB; IstR-1; Persisters; CRISPR-Cas; CRISPR-Cas adaptation reporter; Escherichia coli; Biologi med inriktning mot mikrobiologi; Biology with specialization in Microbiology;

    Sammanfattning : It is nowadays widely accepted that non-coding RNAs play important roles in post-transcriptional regulation of genes in all kingdoms of life. In bacteria, the largest group of RNA regulators are the small RNAs (sRNAs). LÄS MER

  4. 3. Multifaceted RNA-mediated regulatory mechanisms in Streptococcus pyogenes

    Författare :Anaïs Le Rhun; Emmanuelle Charpentier; Franz Narberhaus; Umeå universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Streptococcus pyogenes; small RNAs; CRISPR; Cas9; tracrRNA; RNases; gene expression; RNA sequencing; Infectious Diseases; infektionssjukdomar; mikrobiologi; Microbiology;

    Sammanfattning : Bacterial pathogens rely on precise regulation of gene expression to coordinate host infection processes and resist invasion by mobile genetic elements. An interconnected network of protein and RNA regulators dynamically controls the expression of virulence factors using a variety of mechanisms. LÄS MER

  5. 4. Investigating novel mechanisms for transcriptional memory using genetic engineering

    Författare :Yerma Pareja Sanchez; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Transcriptional memory is phenomenon that has gained interest due to its potential impact on human health. With our work, we wanted to contribute to the understanding of its regulation. To do so, we performed genome-wide knockout screens in both budding yeast and human cells. LÄS MER

  7. 5. Novel diagnostic methods for rapid characterization of extended-spectrum beta-lactamase- and carbapenemase-producing Enterobacteriaceae

    Författare :Muhammad Humaun Kabir; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Klebsiella pneumoniae and Escherichia coli are two of the most clinically important pathogens of the family Enterobacteriaceae. Both can cause severe infections includ-ing urinary tract infections (UTI), bloodstream infections (BSI) and pneumonia. Both are also involved in dissemination of antibiotic resistance. LÄS MER