Sökning: "groove-binding"

Visar resultat 1 - 5 av 12 avhandlingar innehållade ordet groove-binding.

  1. 1. Groove-binding Unsymmetrical Cyanine Dyes for DNA Applications

    Författare :Maja Eriksson; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; polarized spectroscopy; bacteriophage T5; cryo-transmission electron microscopy; dynamic light scattering; DNA; intercalation; cyanine dyes; groove-binding; fluorescence spectroscopy; gel electrophoresis;

    Sammanfattning : This work focuses on interactions between DNA and a group of newly developed unsymmetrical cyanine dyes. The aim of the work was to discover fluorescent dyes that can be used in biophysical studies of natural DNA where minimum perturbation of the DNA-helix is essential, where a slow dissociation rate of the dyes is needed and where the ionic strength is an important parameter. LÄS MER

  3. 2. DNA - drug interactions : groove binding versus intercalation

    Författare :Ulrica Sehlstedt; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

    Sammanfattning : .... LÄS MER

  4. 3. Detection of Biomolecules: Syntheses and Studies of Minor Groove Binding Unsymmetrical Cyanine Dyes and Chromogenic Enzyme Substrates

    Författare :Jonas Karlsson; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; palladium-catalysed cross-couplings; optical spectroscopy; minor groove binding; DNA; cyanine dye; enzyme; intercalation; fluorescence; chromogenic;

    Sammanfattning : Visualisation or detection of biomolecules is usually achieved by the use of fluorescent or highly coloured chromophores. The work presented in this thesis deals with the development of new chromogenic substrates for the detection of enzyme activity, and new DNA-binding fluorescent dyes for staining of DNA. LÄS MER

  5. 4. Minor groove binding unsymmetrical cyanine dyes : binding and dissociation studies in free solution and in electrophoresis gels

    Författare :Maja Eriksson; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; electrophoresis; cyanide dyes; dissociation kinetics; minor groove binding; DNA; stopped-flow; unwinding; mobility shift assays;

    Sammanfattning : .... LÄS MER

  7. 5. DNA-groove binding studied with polarized-light spectroscopy

    Författare :Karl Jansen; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

    Sammanfattning : .... LÄS MER