Sökning: "enzyme substrate"

Visar resultat 16 - 20 av 508 avhandlingar innehållade orden enzyme substrate.

  1. 16. The Importance of Being Promiscuous : Understanding enzyme function, specificity, and evolution through structure

    Författare :Annika Söderholm; Maria Selmer; Birte Höcker; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Enzyme evolution; enzyme promiscuity; X-ray crystallography.;

    Sammanfattning : Enzymes are known to be amazingly specific and efficient catalysts. However, many enzymes also have so-called promiscuous functions, i.e., they are able to catalyze other reactions than their main one. LÄS MER

  3. 17. Theoretical modeling of metal- and enzyme catalyzed transformations

    Författare :Jonas Nyhlén; Jan-Erling Bäckvall; Timofei Privalov; Walter Thiel; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; density functinal theory; computational chemistry; directed evolution; enzyme; mechanistic studies; catalysis; ruthenium; hydrogen transfer; racemization; artificial photosynthesis; frustrated lewis pairs; hydrogen storage; Organic chemistry; Organisk kemi; organisk kemi; Organic Chemistry;

    Sammanfattning : This thesis is focused on describing and predicting catalytic reactions. The major part of the work is based on density functional theory (DFT). In some cases where the size of the investigated system precluded the use of more accurate methods molecular dynamics was employed. LÄS MER

  4. 18. Substrate specificity of protein kinases studied with synthetic peptides

    Författare :Reet Toomik; Uppsala universitet; []
    Nyckelord :Medical sciences; protein kinase A; protein kinase C; substrate; peptide; specificity; stereospecificity; assay; SPOTS peptides; MEDICIN OCH VÅRD; MEDICINE; MEDICIN; Medical and Physiological Chemistry; medicinsk och fysiologisk kemi;

    Sammanfattning : This thesis concentrates on the substrate specificities of protein kinases A and C (PKA, PKC). Synthetic peptides are used for that purpose, both in soluble and cellulose membrane-bound form. The, stereospecificity of both protein kinases was studied with D-amino acid containing synthetic peptides. LÄS MER

  5. 19. Engineering Candida antarctica Lipase A for Enantioselective Transformations in Organic Synthesis : Design, Immobilization and Organic Solvent Screening of Smart Enzyme Libraries

    Författare :Ylva Wikmark; Jan-Erling Bäckvall; Romas Kazlauskas; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Candida antarctica Lipase A; protein engineering; enzyme libraries; enzyme immobilization; biocatalysis; organisk kemi; Organic Chemistry;

    Sammanfattning : The use of enzymes as catalysts in organic synthesis constitutes an attractive alternative to conventional chemical catalysis. Enzymes are non-toxic and biodegradable and they can operate under mild reaction conditions. LÄS MER

  7. 20. Modulating Enzyme Functions by Semi-Rational Redesign and Chemical Modifications : A Study on Mu-class Glutathione Transferases

    Författare :Malena A Norrgård; Bengt Mannervik; Birgitta Tomkinson; Timothy Mantle; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; protein redesign; semi-rational redesign; saturation mutagenesis; iterative saturation mutagenesis; chemical modification; Cys; Cys-X scanning; enzyme evolution; promiscuous; substrate selectivity; enantioselectivity; Biochemistry; Biokemi; Biokemi; Biochemistry;

    Sammanfattning : Today, enzymes are extensively used for many industrial applications, this includes bulk and fine-chemical synthesis, pharmaceuticals and consumer products. Though Nature has perfected enzymes for many millions of years, they seldom reach industrial performance targets. LÄS MER