Sökning: "enzyme substrate"

Visar resultat 11 - 15 av 508 avhandlingar innehållade orden enzyme substrate.

1. 11. Enzyme assays for lignin–carbohydrate bond hydrolysis

   Författare :Hampus Sunner; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Enzyme kinetic param- eter estimation; Glucuronoyl esterase; Enzyme assays; Lignin-carbohydrate bonds; Synergistic enzyme assay;

   Sammanfattning : To identify, produce, and use enzymes, analytical methods known as enzyme assays are employed. Enzyme assays are based on analysing the changes brought about on a substrate by an enzyme under defined conditions. Assays are often based on simplified reactions acting as substitutes for the reaction of interest. LÄS MER

3. 12. Protein Engineering of Candida antarctica Lipase A : Enhancing Enzyme Properties by Evolutionary and Semi-Rational Methods

   Författare :Anders G. Sandström; Jan-Erling Bäckvall; Uwe T. Bornscheuer; Stockholms universitet; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; lipase; protein engineering; directed evolution; kinetic resolution; structural biology; Enzyme engineering; Enzymteknik; organisk kemi; Organic Chemistry;

   Sammanfattning : Enzymes are gaining increasing importance as catalysts for selective transformations in organic synthetic chemistry. The engineering and design of enzymes is a developing, growing research field that is employed in biocatalysis. LÄS MER

4. 13. Towards Classification and Functional Description of Enzymes: A case study of feruloyl esterases

   Författare :Gupta Udatha; Chalmers tekniska högskola; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; descriptors; enzyme immobilization; catalytic triad; pharmacophore; enzyme promiscuity; functional classification; molecular docking; feruloyl esterases; protein evolution; structure-function relationship;

   Sammanfattning : The prediction of enzyme functionality from sequence or structure data remains a challenging task that can be best addressed by studying the structure-function relationships determined from previously available information. This thesis work was focused on developing a reliable classification and functional description for the feruloyl esterase (FAE) enzyme family, whose members’ possess both structural and catalytic promiscuity. LÄS MER

5. 14. Molecular Simulation of Enzyme Catalysis and Inhibition

   Författare :Sinisa Bjelic; Johan Åqvist; Adrian J. Mulholland; Uppsala universitet; []
   Nyckelord :Theoretical chemistry; enzyme catalysis; enzyme inhibition; computer simulations; molecular dynamics; empirical valence bond method; structure-based inhibitor design; Teoretisk kemi;

   Sammanfattning : The reaction mechanisms for the hemoglobin degrading enzymes in the Plasmodium falciparum malaria parasite, plasmepsin II (Plm II) and histo-aspartic protease (HAP), have been analyzed by molecular simulations. The reaction free energy profiles, calculated by the empirical valence bond (EVB) method in combination with molecular dynamics (MD) and free energy perturbation (FEP) simulations are in good agreement with experimental data. LÄS MER

7. 15. Exploiting enzyme promiscuity for rational design

   Författare :Cecilia Branneby; Karl Hult; Alex Zaks; KTH; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biochemistry; Candida antarctica lipase B; alanine racemase; substrate specificity; reaction specificity; enzyme catalytic promiscuity; Biokemi; Biochemistry; Biokemi;

   Sammanfattning : Enzymes are today well recognized in various industrial applications, being an important component in detergents, and catalysts in the production of agrochemicals, foods, pharmaceuticals, and fine chemicals. Their large use is mainly due to their high selectivity and environmental advantage, compared to traditional catalysts. LÄS MER