Sökning: "affinity chromatography"
Visar resultat 1 - 5 av 128 avhandlingar innehållade orden affinity chromatography.
1. Affinity-, Partition- and Permeability Properties of the Human Red Blood Cell Membrane and Biomembrane Models, with Emphasis on the GLUT1 Glucose Transporter
Sammanfattning : The human glucose transporter GLUT1 is abundant in red blood cells, the blood-brain barrier and epithelial cells, where it mediates the transport of the energy metabolite, glucose. In the present work some properties of GLUT1, including affinity binding of both substrates and inhibitors, transport rates as well as permeabilities of aromatic amino acids and drug-membrane interactions were analyzed by chromatographic methods. LÄS MER
2. Chromatographic Studies of Solute Interactions with Immobilized Red Blood Cells and Biomembranes
Sammanfattning : Specific and non-specific interactions of solutes with immobilized biomembranes were studied using chromatographic methods. Liposomes, proteoliposomes and red blood cell (RBC) membrane vesicles were immobilized by a freeze-thawing procedure, whereas whole RBCs were adsorbed in the gel beds using electrostatic interaction, binding to wheat germ agglutinin (WGA) or the streptavidin-biotin interaction. LÄS MER
3. Protein engineering to explore and improve affinity ligands
Sammanfattning : In order to produce predictable and robust systems forprotein purification and detection, well characterized, small,folded domains descending from bacterial receptors have beenused. These bacterial receptors, staphylococcal protein A (SPA)and streptococcal protein G (SPG), possess high affinity to IgGand / or HSA. LÄS MER
4. Fast isolation of proteins from bioreactors using affinity chromatography techniques
Sammanfattning : The purification of proteins from raw materials like fermentation broth and other biological sources usually involves several purification steps and is rather time consuming and expensive. This is a major concern in the production of many important protein products e.g. pharmaceuticals such as insulin and blood coagulation factors. LÄS MER
5. Insights into weak affinity antibody-antigen interactionsStudies using affinity chromatography and optical biosensor
Sammanfattning : Molecular recognition and interaction is fundamental for the function of biological systems. The properties of the interacting biomolecules dictate the type of forces involved and the strength and dynamics of the interaction. Many interactions are very strong whereas others exhibit weak affinity. LÄS MER
