Sökning: "Protein recognition"

Visar resultat 1 - 5 av 392 avhandlingar innehållade orden Protein recognition.

1. 1. Molecular principles of protein stability and protein-protein interactions

   Författare :Christofer Lendel; Per-Åke Nygren; Christopher M. Dobson; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; affibody; binding thermodynamics; induced fit; molten globule; NMR spectroscopy; protein engineering; protein-protein interactions; protein stability; protein structure.; Structural biochemistry; Strukturbiokemi;

   Sammanfattning : Proteins with highly specific binding properties constitute the basis for many important applications in biotechnology and medicine. Immunoglobulins have so far been the obvious choice but recent advances in protein engineering have provided several novel constructs that indeed challenge antibodies. LÄS MER

3. 2. Protein–DNA Recognition : In Vitro Evolution and Characterization of DNA-Binding Proteins

   Författare :Mikael Nilsson; Mikael Widersten; Dan Tawfik; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; In vitro evolution; Phage display; mRNA display; Cro; DNA recognition; repressor; Biokemi; Biochemistry; Biokemi;

   Sammanfattning : DNA-recognizing proteins are involved in a multitude of important life-processes. Therefore, it is of great interest to understand the underlying mechanisms that set the rules for sequence specific protein–DNA interactions. Previous attempts aiming to resolve these interactions have been focused on naturally occurring systems. LÄS MER

4. 3. Structural and thermodynamical basis for molecular recognition between engineered binding proteins

   Författare :Jakob Dogan; Per-Åke Nygren; John E. Ladbury; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; protein structure; induced fit; binding thermodynamics; NMR spectroscopy; protein engineering; protein-protein interactions; protein stability; calorimetry; Structural biochemistry; Strukturbiokemi;

   Sammanfattning : The structural determination of interacting proteins, both as individual proteins and in their complex, complemented by thermodynamical studies are vital in order to gain in-depth insights of the phenomena leading to the highly selective protein-protein interactions characteristic of numerous life processes. This thesis describes an investigation of the structural and thermodynamical basis for molecular recognition in two different protein-protein complexes, formed between so-called affibody proteins and their respective targets. LÄS MER

5. 4. Protein modularity : Structure and interactions by NMR and SPR

   Författare :Amélie Wallenhammar; Maria Sunnerhagen; Björn Wallner; Lotta Tollstoy-Tegler; Helena Berglund; Linköpings universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

   Sammanfattning : Protein degradation is a fundamental cornerstone of regulation of protein levels in the human body. For proper function, numerous molecular processes rely on fine-tuned communication through specific interactions between proteins. LÄS MER

7. 5. Structural Transitions in Helical Peptides : The Influence of Water – Implications for Molecular Recognition and Protein Folding

   Författare :Martin Lignell; Hans-Christian Becker; Kaare Teilum; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; protein dynamics; protein folding; molten globule; time-resolved fluorescence spectroscopy; CD spectroscopy; molecular recognition; structure-function paradigm; Chemical physics; Kemisk fysik; Physical Chemistry; Fysikalisk kemi;

   Sammanfattning : Fluctuations in protein structure are vital to function. This contrasts the dominating structure-function paradigm, which connects the well-defined three-dimensional protein structure to its function. However, catalysis is observed in disordered enzymes, which lack a defined structure. LÄS MER