Sökning: "mRNA display"

Visar resultat 1 - 5 av 73 avhandlingar innehållade orden mRNA display.

  1. 1. Protein–DNA Recognition : In Vitro Evolution and Characterization of DNA-Binding Proteins

    Författare :Mikael Nilsson; Mikael Widersten; Dan Tawfik; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; In vitro evolution; Phage display; mRNA display; Cro; DNA recognition; repressor; Biokemi; Biochemistry; Biokemi;

    Sammanfattning : DNA-recognizing proteins are involved in a multitude of important life-processes. Therefore, it is of great interest to understand the underlying mechanisms that set the rules for sequence specific protein–DNA interactions. Previous attempts aiming to resolve these interactions have been focused on naturally occurring systems. LÄS MER

  3. 2. Expression and Mutation Analyses of Candidate Cancer Genes In Situ

    Författare :Sara Kiflemariam; Tobias Sjöblom; Fredrik Pontén; Mef Nilbert; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; cancer; in situ; in situ hybridization; padlock probes; mRNA; tyrosine kinases; tyrosine phosphatases; fusion transcripts;

    Sammanfattning : Cancers display heterogeneity in genetic profiles of the individual cancer cells and in the composition of different malignant and non-malignant cell populations. Such intra-tumor heterogeneity plays a role in treatment response and the emergence of resistance to cancer therapies. LÄS MER

  4. 3. Respiratory NAD(P)H dehydrogenases of plants - Gene identity and expression in response to light and cold

    Författare :Staffan Svensson; Biologiska institutionen; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; morfologi; phytogeography; chemotaxonomy. Physiology of nonvascular plants; Systematisk botanik; taxonomi; morphology; taxonomy; Systematic botany; Växtgenetik; Plant genetics; plant mitochondria; NADH dehydrogenases; light dependence; mRNA quantification; leaf respiration; cold treatment; kemotaxonomi. Växters fysiologi inte kärlväxter .;

    Sammanfattning : The respiratory chain of plants contains class 2 NAD(P)H dehydrogenases, not present in animals. The functional roles of these enzymes have been elusive for a long time. Two cDNAs, homologous to class 2 NADH dehydrogenase genes of yeast and E. coli, were isolated from potato leaves. LÄS MER

  5. 4. Molecular mechanisms involved in glioma cell interactions in vitro and studies of PDGF B transcript variants

    Författare :Susanne Heller; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; Tumor heterogeneity; glioma; differential display; PDGF B; Genetik; Clinical genetics; Klinisk genetik; Pathology; patologi;

    Sammanfattning : Glioblastoma multiforme is a malignant brain tumor characterized by heterogeneity.Interactions between heterogeneous tumor cells are supposed to affect the behavior of awhole tumor cell population. LÄS MER

  7. 5. Rational and combinatorial genetic engineering approaches for improved recombinant protein production and purification

    Författare :Nina Bandmann; Per-Åke Nygren; Helena Berglund; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Aqueous two-phase system; combinatorial library; expression vector; flow cytometry; fusion tag; partitioning; peptide library; phage display; recombinant proteins; ribosomal binding site; translation initiation; Bioengineering; Bioteknik;

    Sammanfattning : The bacterium Escherichia coli (E. coli) is in many situations an ideal host for production of recombinant proteins, since it generally provides a rapid and economical means to achieve sufficiently high product quantities. LÄS MER