Sökning: "DNA recognition"

Visar resultat 1 - 5 av 169 avhandlingar innehållade orden DNA recognition.

  1. 1. Protein–DNA Recognition : In Vitro Evolution and Characterization of DNA-Binding Proteins

    Författare :Mikael Nilsson; Mikael Widersten; Dan Tawfik; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; In vitro evolution; Phage display; mRNA display; Cro; DNA recognition; repressor; Biokemi; Biochemistry; Biokemi;

    Sammanfattning : DNA-recognizing proteins are involved in a multitude of important life-processes. Therefore, it is of great interest to understand the underlying mechanisms that set the rules for sequence specific protein–DNA interactions. Previous attempts aiming to resolve these interactions have been focused on naturally occurring systems. LÄS MER

  3. 2. Development of benzoquinoquinoxaline derivatives as triplex-specific probes : Recognition of DNA structures at repeats sequences

    Författare :Helen Bergquist; Rula Zain; Keith Fox; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Triplex; H-DNA; BQQ; BQQ-OP; BQQ-Bodipy; triplet repeat; DNA; non-B-DNA; pkd1; frataxin; Friedreich s ataxia; Molecular biology; Molekylärbiologi; Molecular Biology; molekylärbiologi;

    Sammanfattning : Repeat sequences are associated with several human diseases, such as Friedreich’s ataxia, polycystic kidney disease and cancer. These sequences can form non-B-DNA structures, including triplex (H-DNA) DNA, and are associated with genomic instability and altered gene expression. LÄS MER

  4. 3. Fixation of Functionalized DNA Nanostructures

    Författare :Erik Lundberg; Chalmers tekniska högskola; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; triplex recognition; dna; self-assembly; gel electrophoresis; nanotechnology; cross-linking; spectroscopy; fixation; fret; click chemistry;

    Sammanfattning : This thesis features results from two different studies on a fully addressable non-repetitive network on the nanometre scale, assembled by synthetic DNA molecules. A bio-inspired molecular approach, based on principles of self-assembly, has brought about a paradigm shift in nanoscience, from a top-down to a bottom-up perspective. LÄS MER

  5. 4. Bottom-up Fabrication of Functional DNA Nanostructures

    Författare :Erik Lundberg; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; click chemistry; gel electrophoresis; self-assembly; cross-linking; lipid membrane; fixation; linear dichroism; nanotechnology; spectroscopy; fret; dna; triplex recognition;

    Sammanfattning : This thesis demonstrates bottom-up fabrication of a fully addressable non-repetitive network on the nanometer scale, assembled by synthetic DNA molecules. Each side constitutes a unique sequence of 10 bases, i.e. 3. LÄS MER

  7. 5. Probing Biomolecular Recognition at the Single Molecule Level

    Författare :Anders Gunnarsson; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; cell membrane; TIRFM; liposome; time-of-flight secondary ion mass spectrometry; membrane receptor; single molecule; TOF-SIMS; molecular recognition; DNA; barcode; total internal reflection; multiplexing;

    Sammanfattning : Specific recognition between biomolecular partners contributes to a multitude of biochemical signaling within and between cells. The aim of this work has been to investigate the possibility to probe biomolecular recognition reactions at the smallest possible scale, the single molecule level. LÄS MER