Sökning: "Live-Cell Imaging"

Visar resultat 1 - 5 av 39 avhandlingar innehållade orden Live-Cell Imaging.

1. 1. Algorithmic discovery, development and personalized selection of higher-order drug cocktails : A label-free live-cell imaging & secretomics approach

   Författare :Efthymia Chantzi; Mats G Gustafsson; Ulf Hammerling; Andreas Bender; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; drug combination discovery and development; personalized pharmacotherapy; higher-order drug combination analysis; quantitative label-free live-cell imaging; secretomics; cell-cell communication; deep learning; convolutional neural networks; data mining; MapReduce; resampling; generalized highest single agent; COMBImageDL; COMBSecretomics; Bioinformatics; Bioinformatik; Pharmacology; Farmakologi; Engineering Science; Teknisk fysik;

   Sammanfattning : An upward trend in clinical pharmacology is the use of multiple drugs to combat complex and co-occurring diseases due to better efficacy, decreased toxicity and reduced risk of evolving resistance. Despite high late-stage attrition rates and the need for multi drug treatments, most drug discovery and development efforts are still mainly focused on new one-size-fits-all monotherapies. LÄS MER

3. 2. Automating STED microscopy for functional and structural live-cell imaging

   Författare :Jonatan Alvelid; Ilaria Testa; Eggeling Christian; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; STED; microscopy; nanoscopy; super-resolution microscopy; automation; image analysis; fluorophores; Biologisk fysik; Biological Physics;

   Sammanfattning : Optical microscopy imaging methods are today invaluable tools for studies in life sciences as they allow visualization of biological systems, tissues, cells, and sub-cellular compartments from millimetres down to nanometres. The invention and development of nanoscopy in the past 20 years has pushed fluorescence microscopy down to the nanoscale, reaching beyond the natural diffraction limit of light that does not allow focusing of visible light below sizes of around 200 nm, and into the realm of what was previously only thought possible with electron microscopy. LÄS MER

4. 3. Imaging Living Cells with an X-ray Laser

   Författare :Gijs van der Schot; Tomas Ekeberg; Janos Hajdu; Filipe Maia; Pierre Thibault; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Coherent diffractive X-ray imaging; flash X-ray imaging; lensless imaging; single particle imaging; cyanobacteria; phasing; image classification; substrate-free sample delivery; X-ray free-electron laser; XFEL; FEL; CDXI; CDI; CXI; FXI; Chemistry with specialization in Biophysics; Kemi med inriktning mot biofysik;

   Sammanfattning : Imaging living cells at a resolution higher than the resolution of optical microscopy is a significant challenge. Fluorescence microscopy can achieve a degree of super-resolution via labeling cellular components with a fluorescent dye. LÄS MER

5. 4. Sugar-modulated gene expression and cell division in cell culture and seedlings of A. thaliana

   Författare :Sabine Kunz; Leszek A. Kleczkowski; Alison Smith; Umeå universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Arabidopsis thaliana; carbohydrates; cell culture; cell division; gene expression; homeostasis; live cell imaging; sugar-signals; sugar-analogues; Physiological Botany; fysiologisk botanik;

   Sammanfattning : Throughout their life cycle, plants adjust growth in response to their developmental and environmental situation within the limits of their energetic capacities. This capacity is defined by the local sugar availability, which is constantly modulated through synthesis, transport and consumption of sugar. LÄS MER

7. 5. Development of quantitative optical microscopy methods for single cell analysis

   Författare :Katarina Logg; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; protein localization; quantitative optical microscopy; live cell imaging; image analysis; cellular stress;

   Sammanfattning : Optical microscopy has brought powerful methods to biological research, in particular the possibility to assay dynamic molecular processes within living individual cells. This thesis deals with various aspects of fluorescence and Raman microscopy, such as the possibility of avoiding cellular damage or stress caused by illumination, how to manipulate single cells for spectroscopic analysis and how to quantify images automatically. LÄS MER