Sökning: "STED"

Visar resultat 1 - 5 av 17 avhandlingar innehållade ordet STED.

  1. 1. Skolen finder sted

    Författare :Inge Mette Kirkeby; Katja Grillner; Sten Gromark; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; school buildings; pedagogy; interaction; Architecture; Arkitektur;

    Sammanfattning : Målet med forskningsprojektet "Skolen finder sted" er at etablere viden om samspillet mellem pædagogik og arkitektur, mellem børn og rum, og om samspillets beskaffenhed. En sådan viden forventes at kunne medvirke til at kvalificere dialog og beslutningsgrundlag i kommende skolebyggerier samt indgå i arkitektens værktøjskasse, projektets overordnede vinkling er således designteoretisk. LÄS MER

  3. 2. Automating STED microscopy for functional and structural live-cell imaging

    Författare :Jonatan Alvelid; Ilaria Testa; Eggeling Christian; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; STED; microscopy; nanoscopy; super-resolution microscopy; automation; image analysis; fluorophores; Biologisk fysik; Biological Physics;

    Sammanfattning : Optical microscopy imaging methods are today invaluable tools for studies in life sciences as they allow visualization of biological systems, tissues, cells, and sub-cellular compartments from millimetres down to nanometres. The invention and development of nanoscopy in the past 20 years has pushed fluorescence microscopy down to the nanoscale, reaching beyond the natural diffraction limit of light that does not allow focusing of visible light below sizes of around 200 nm, and into the realm of what was previously only thought possible with electron microscopy. LÄS MER

  4. 3. Investigation of Neuronal Protein Trafficking at the Molecular Scale

    Författare :Martina Damenti; Ilaria Testa; Clive Bramham; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Endoplasmic Reticulum; Mitochondria; Arc Activity-regulated cytoskeleton-associated protein ; oligomerization; pRESOLFT; STED; event-triggered FCS; DNA-PAINT; STARSS; molecular dynamics simulations; Biological and Biomedical Physics; Biologisk och biomedicinsk fysik;

    Sammanfattning : Neurons are polarized cells that encode information in the nervous system viaelectrochemical connections named synapses. The tuning of synapticconnections is enabled by a plastic protein trafficking system which operates atthe nanoscale to finely tweak the neuronal ultrastructure. LÄS MER

  5. 4. Super resolution optical imaging – image analysis, multicolor development and biological applications

    Författare :Daniel Rönnlund; Jerker Widengren; Christian Eggeling; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Stimulated emission depletion STED microscopy; nanoscopy; multicolor; image analysis; diagnostics; cancer; metastasis; Biological Physics; Biologisk fysik;

    Sammanfattning : This thesis focuses on super resolution STED optical imaging. STED provides a wealth of new informational content to the acquired images by using stimulated emission to surpass the diffraction limit in optical fluorescence microscopy. LÄS MER

  7. 5. Super resolution fluorescence imaging : analyses, simulations and applications

    Författare :Jan Bergstrand; Jerker Widengren; Eilon Sherman; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Physics; Fysik;

    Sammanfattning : Fluorescence methods offer extraordinary sensitivity and specificity, and are extensively used in the life sciences. In recent years, super resolution fluorescence imaging techniques have developed strongly, uniquely combining ~10 nm sub diffraction resolution and specific labeling with high efficiency. LÄS MER