Sökning: "nanoscopy"

Hittade 3 avhandlingar innehållade ordet nanoscopy.

  1. 1. Automated super-resolution microscopy for high-throughput imaging

    Författare :Xavier Casas Moreno; Ilaria Testa; Florian Jug; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; RESOLFT; throughput; microscopy automation; nanoscopy; Biologisk fysik; Biological Physics;

    Sammanfattning : Fluorescence microscopes enable the visualization of biological samples with high contrast by labeling specific structures with fluorescent molecules. However, the spatial resolution of widely used microscopy techniques, such as widefield and confocal microscopy, is limited by the size of a focused spot of light, which roughly corresponds to half the wavelength of the illumination. LÄS MER

  3. 2. Automating STED microscopy for functional and structural live-cell imaging

    Författare :Jonatan Alvelid; Ilaria Testa; Eggeling Christian; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; STED; microscopy; nanoscopy; super-resolution microscopy; automation; image analysis; fluorophores; Biologisk fysik; Biological Physics;

    Sammanfattning : Optical microscopy imaging methods are today invaluable tools for studies in life sciences as they allow visualization of biological systems, tissues, cells, and sub-cellular compartments from millimetres down to nanometres. The invention and development of nanoscopy in the past 20 years has pushed fluorescence microscopy down to the nanoscale, reaching beyond the natural diffraction limit of light that does not allow focusing of visible light below sizes of around 200 nm, and into the realm of what was previously only thought possible with electron microscopy. LÄS MER

  4. 3. Super resolution optical imaging – image analysis, multicolor development and biological applications

    Författare :Daniel Rönnlund; Jerker Widengren; Christian Eggeling; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Stimulated emission depletion STED microscopy; nanoscopy; multicolor; image analysis; diagnostics; cancer; metastasis; Biological Physics; Biologisk fysik;

    Sammanfattning : This thesis focuses on super resolution STED optical imaging. STED provides a wealth of new informational content to the acquired images by using stimulated emission to surpass the diffraction limit in optical fluorescence microscopy. LÄS MER