Sökning: "single particle imaging"
Visar resultat 1 - 5 av 55 avhandlingar innehållade orden single particle imaging.
1. Structural Studies of Large dsDNA Viruses using Single Particle Methods
Sammanfattning : Structural studies of large biological assemblies pose a unique problem due to their size, complexity and heterogeneity. Conventional methods like x-ray crystallography, NMR, etc. are limited in their ability to address these issues. To overcome some of these limitations, single particle methods were used. LÄS MER
2. Coherent Diffractive Imaging with X-ray Lasers
Sammanfattning : The newly emerging technology of X-ray free-electron lasers (XFELs) has the potential to revolutionise molecular imaging. XFELs generate very intense X-ray pulses and predictions suggest that they may be used for structure determination to atomic resolution even for single molecules. LÄS MER
3. Single Molecule Detection : Microfluidic Automation and Digital Quantification
Sammanfattning : Much of recent progress in medical research and diagnostics has been enabled through the advances in molecular analysis technologies, which now permit the detection and analysis of single molecules with high sensitivity and specificity. Assay sensitivity is fundamentally limited by the efficiency of the detection method used for read-out. LÄS MER
4. Statistical processing of Flash X-ray Imaging of protein complexes
Sammanfattning : Flash X-ray Imaging (FXI) at X-ray Free Electron Lasers (XFELs) is a promising technique that permits the investigation of the 3D structure of molecules without the need for crystallization, by diffracting on single individual sample particles.In the past few years, some success has been achieved by using FXI on quite large biological complexes (40 nm-1 μm in diameter size). LÄS MER
5. Imaging Living Cells with an X-ray Laser
Sammanfattning : Imaging living cells at a resolution higher than the resolution of optical microscopy is a significant challenge. Fluorescence microscopy can achieve a degree of super-resolution via labeling cellular components with a fluorescent dye. LÄS MER