Sökning: "spacer integration"

Visar resultat 1 - 5 av 11 avhandlingar innehållade orden spacer integration.

  1. 1. The type I-E CRISPR-Cas system : Biology and applications of an adaptive immune system in bacteria

    Författare :Lina Amlinger; Magnus Lundgren; Peter Fineran; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; CRISPR; CRISPR-Cas; virus defense; bacteria; bacteriophage; adaptation; spacer integration; interference; gene silencing; fluorescent reporter; Escherichia coli; Mikrobiologi; Microbiology;

    Sammanfattning : CRISPR-Cas systems are adaptive immune systems in bacteria and archaea, consisting of a clustered regularly interspaced short palindromic repeats (CRISPR) array and CRISPR associated (Cas) proteins. In this work, the type I-E CRISPR-Cas system of Escherichia coli was studied.CRISPR-Cas immunity is divided into three stages. LÄS MER

  3. 2. Integration of epitaxial SiGe(C) layers in advanced CMOS devices

    Författare :Julius Hållstedt; Henry Radamson; Eugene A. Fitzgerald; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Silicon Germanium Carbon SiGeC ; Chemical Vapor Deposition CVD ; Epitaxy; Pattern Dependency; MOSFET; Mobility; Spacer Gate Technology; Semiconductor physics; Halvledarfysik;

    Sammanfattning : Heteroepitaxial SiGe(C) layers have attracted immense attention as a material for performance boost in state of the art electronic devices during recent years. Alloying silicon with germanium and carbon add exclusive opportunities for strain and bandgap engineering. LÄS MER

  4. 3. Process integration issues for high-performance bipolar technology

    Författare :Tord E. Karlin; KTH; []
    Nyckelord :;

    Sammanfattning : The work in this thesis has been focused on processintegration issues for high-performance bipolar technologyincluding experimental work on self-aligned silicides,ion-implanted andin situdoped polysilicon emitters, strained silicongermanium for heterojunction bipolar transistors and physicalprocess and device simulation.Key issues for the self-aligned silicidation of small devicefeatures such as the influence of dopants, silicon morphologyand line width on titanium disilicide formation, phasetransformation and temperature stability, have been addressed. LÄS MER

  5. 4. Source and drain engineering in SiGe-based pMOS transistors

    Författare :Christian Isheden; Mikael Östling; Simon Deleonibus; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Electronics; SiGe; source drain; shallow junctions; pMOS; process integration; CVD; epitaxy; etching; Ni silicide; contact resistivity; Elektronik; Electronics; Elektronik;

    Sammanfattning : A new shallow junction formation process, based on selective silicon etching followed by selective growth of in situ B-doped SiGe, is presented. The approach is advantageous compared to conventional ion implantation followed by thermal activation, because perfectly abrupt, low resistivity junctions of arbitrary depth can be obtained. LÄS MER

  7. 5. Small RNAs, Big Consequences : Post-transcriptional Regulation and Adaptive Immunity in Bacteria

    Författare :Mirthe Hoekzema; Gerhart E. H. Wagner; Magnus Lundgren; Kai Papenfort; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; small RNA; sRNA; non-coding RNA; Hfq; gene regulation; post-transcriptional regulation; biofilm; OmrA; OmrB; toxin-antitoxin; TisB; IstR-1; Persisters; CRISPR-Cas; CRISPR-Cas adaptation reporter; Escherichia coli; Biologi med inriktning mot mikrobiologi; Biology with specialization in Microbiology;

    Sammanfattning : It is nowadays widely accepted that non-coding RNAs play important roles in post-transcriptional regulation of genes in all kingdoms of life. In bacteria, the largest group of RNA regulators are the small RNAs (sRNAs). LÄS MER