Sökning: "sequence-specific binding"

Visar resultat 1 - 5 av 32 avhandlingar innehållade orden sequence-specific binding.

1. 1. Thermodynamics of Peptide Nucleic Acid Interactions with DNA

   Författare :Tommi Ratilainen; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; thermodynamics; enthalpy-entropy compensation; ionic strength; nucleic acids; fluorescence energy transfer; PNA; calorimetry; sequence-specific binding;

   Sammanfattning : Peptide Nucleic Acid (PNA) is a DNA mimic with the natural nucleobases attached to a charge-neutral pseudopeptide backbone. It has remarkably strong and sequence-specific binding affinity to natural nucleic acids (DNA and RNA), mainly attributed to the lack of charge in the PNA backbone. LÄS MER

3. 2. Unravelling Mechanistic Insights of Eukaryotic Transcriptional Control

   Författare :Johanna Hörberg; Göteborgs universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Transcriptional control; Gene expression regulation; DNA supercoiling; DNA methylation; DNA-protein recognition; Basic-Leucine-Zipper transcription factors; Basic-Helix-Loop-Helix transcription factors; Sequence specific DNA plasticity; Transcription factor cooperativity; Cancer; Molecular modelling; Molecular dynamics;

   Sammanfattning : DNA transcription involves the association of multiple proteins with DNA to convert the genetic information into messenger-RNA transcripts, which later will be translated into proteins. Con-trolled regulation of transcription is imperative for preserving the healthy and homeostatic state of cells. LÄS MER

4. 3. Competitive-Binding Based Optical DNA Mapping - From Bacterial Plasmids to the Human Genome

   Författare :Vilhelm Müller; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; genetics; single-molecule; nanofluidics; DNA; antibiotic resistance; optical DNA mapping; plasmids; competitive binding; fluorescence microscopy; bacteria;

   Sammanfattning : Significant advances within the field of DNA sequencing have allowed us to study DNA at a level of detail that was previously impossible. However, dynamic genomic regions with a high degree of structural variations, while being linked to disease in humans and increased resistance to antibiotics, are still challenging to characterize. LÄS MER

5. 4. Determination of transcription factor binding specificities

   Författare :Arttu Jolma; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : The term "genetic code" refers to the way in which the information encoded in nucleic acids is converted into the amino-acid sequence of proteins. There is however also a second genetic code, one that is used by the cells to read the blueprints of the entire organism. LÄS MER

7. 5. Protein–DNA Recognition : In Vitro Evolution and Characterization of DNA-Binding Proteins

   Författare :Mikael Nilsson; Mikael Widersten; Dan Tawfik; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; In vitro evolution; Phage display; mRNA display; Cro; DNA recognition; repressor; Biokemi; Biochemistry; Biokemi;

   Sammanfattning : DNA-recognizing proteins are involved in a multitude of important life-processes. Therefore, it is of great interest to understand the underlying mechanisms that set the rules for sequence specific protein–DNA interactions. Previous attempts aiming to resolve these interactions have been focused on naturally occurring systems. LÄS MER