Sökning: "DNA-protein recognition"

Visar resultat 1 - 5 av 6 avhandlingar innehållade orden DNA-protein recognition.

1. 1. Unravelling Mechanistic Insights of Eukaryotic Transcriptional Control

   Författare :Johanna Hörberg; Göteborgs universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Transcriptional control; Gene expression regulation; DNA supercoiling; DNA methylation; DNA-protein recognition; Basic-Leucine-Zipper transcription factors; Basic-Helix-Loop-Helix transcription factors; Sequence specific DNA plasticity; Transcription factor cooperativity; Cancer; Molecular modelling; Molecular dynamics;

   Sammanfattning : DNA transcription involves the association of multiple proteins with DNA to convert the genetic information into messenger-RNA transcripts, which later will be translated into proteins. Con-trolled regulation of transcription is imperative for preserving the healthy and homeostatic state of cells. LÄS MER

3. 2. Recognition and Visualization of Nucleic Acids

   Författare :Marcus Wilhelmsson; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; intercalation; cyanine dye; ruthenium; polarized spectroscopy; threading; groove-binding; kinetics; genetic screening; DNA; fluorescent base analogue;

   Sammanfattning : The work presented in this Thesis focuses on the interaction between nucleic acids and small molecules, as well as binding or incorporation of reporter chromophores into nucleic acids. The results could help in studying and understanding processes that are fundamental to describe mechanisms behind a genetic disorder. LÄS MER

4. 3. Towards simulating fully charged protein-DNA systems in water : Zif268-DNA as a test case

   Författare :Git Roxström; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Physics; Molecular Dynamics simulation; Zif268; zinc fingers; GROMOS; linkers; recognition sites; Fysik; Physics; Fysik; Physical Chemistry; fysikalisk kemi;

   Sammanfattning : In this thesis, we develop a computing strategy to simulate fully charged protein-DNA systems with a general force field (GROMOS87). The ions neutralizing the phosphates (P-ions) are submitted to global symmetry constraints. A model exhibiting ionic-atmosphere effects on mobile P-ions was implemented via very weak restraint forces. LÄS MER

5. 4. Thermodynamics of protein-DNA interactions

   Författare :Thomas Lundbäck; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :DNA-protein interactions; molecular recognition; thermodynamics; specificity.dehydration; salt dependence.;

   Sammanfattning : This thesis treats the molecular recognition between DNA-binding proteins and DNA and the way these interactions are affected by the surrounding water solution and dissolved solutes. The work involves studies on the thermodynamics of sequence specific DNA-binding of the human glucocorticoid receptor DNA-binding domain and the nonsequence specific DNA- binding of Sso7d from the archaeon Sulfolobus solfataricus. LÄS MER

7. 5. Single-stranded DNA : methods and application in nanotechnology

   Författare :Giulio Bernardinelli; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : Basic molecular and cell research, production of recombinant proteins, diagnostic detection of genetic mutations, construction of nanostructures and high-throughput DNA sequencing are only a few examples of the diverse set of applications that are amenable thanks to the availability of synthetic DNA polymers in biomedicine. Strategic investments and technical progress together with the introduction of automation in the synthesis of DNA oligomers enabled to transform, in just a few decades, a process mastered only by a niche of biochemists into an affordable and available custom-made product to every scientific field. LÄS MER