Sökning: "DNA supercoiling"

Visar resultat 1 - 5 av 8 avhandlingar innehållade orden DNA supercoiling.

  1. 1. Kinetic Recognition of Nucleic Acids - Studies on the DNA Binding Selectivity of Threading Ruthenium Complexes

    Författare :Pär Nordell; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; intercalation; circular dicroism; ruthenium complexes; sequence selectivity; fluorescence; DNA; supercoiling; spectroscopy; kinetics;

    Sammanfattning : Despite the great progress in our understanding of DNA during the past half-century, there are many important aspects of its chemical and biological role yet to be explored. The principles by which it selectively interacts with other molecules have attracted much interest due to the relevance for fundamental cellular processes, as well as for the development of diagnostic probes and effective pharmaceutical agents. LÄS MER

  3. 2. Unravelling Mechanistic Insights of Eukaryotic Transcriptional Control

    Författare :Johanna Hörberg; Göteborgs universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Transcriptional control; Gene expression regulation; DNA supercoiling; DNA methylation; DNA-protein recognition; Basic-Leucine-Zipper transcription factors; Basic-Helix-Loop-Helix transcription factors; Sequence specific DNA plasticity; Transcription factor cooperativity; Cancer; Molecular modelling; Molecular dynamics;

    Sammanfattning : DNA transcription involves the association of multiple proteins with DNA to convert the genetic information into messenger-RNA transcripts, which later will be translated into proteins. Con-trolled regulation of transcription is imperative for preserving the healthy and homeostatic state of cells. LÄS MER

  4. 3. DNA topoisomerases and nucleosome dynamics in fission yeast

    Författare :Ulrika Norman Axelsson; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : The organization of DNA and associated proteins into chromatin allows for compaction and protection of large eukaryotic genomes. However, it also poses a challenge to fundamental cellular processes such as DNA replication, recombination, repair and transcription. LÄS MER

  5. 4. Hypothermia and radiation. Effects on DNA, chromatin and cellular survival

    Författare :Kerstin Elmroth; Göteborgs universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Cells; temperature; irradiation; dsb; survival; fluorescent nucleoid assay; hypothermia;

    Sammanfattning : The effect of ionising radiation on cells can be modulated by several physico-chemical factors. The aim of this study was to investigate if hypothermic (sub-physiological) temperatures (2°C-28°C) may influence the effect of X-rays on cellular damage in human normal fibroblasts and in a human breast adenocarcinoma cell line, MCF-7. LÄS MER

  7. 5. Investigating novel roles of topoisomerase regulatory interactions in transcription

    Författare :Vladislav Kuzin; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Drugs targeting DNA Topoisomerases (TOPs, TOP1 and TOP2) are mainstays of anti-cancer therapy. While they have proven effective, the toxicity of current TOP drugs caused by DNA damage-induced apoptosis of non-cancer cells, limits their use in the clinic. LÄS MER