Sökning: "production of recombinant enzyme"

Visar resultat 6 - 10 av 68 avhandlingar innehållade orden production of recombinant enzyme.

  1. 6. Discovery of Novel Fatty Acid Dioxygenases and Cytochromes P450 : Mechanisms of Oxylipin Biosynthesis in Pathogenic Fungi

    Författare :Inga Hoffmann; Ernst H Oliw; Alan R Brash; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Fusion protein; Linoleate diol synthase; Allene oxide synthase; Cyclooxygenase; Oxygenase; HPLC; Mass spectrometry; Hydroperoxide isomerase; Aspergillus; Fusarium oxysporum; Pharmaceutical Biochemistry; Farmaceutisk biokemi; Farmaceutisk farmakologi; Pharmaceutical Pharmacology; Farmaceutisk vetenskap; Pharmaceutical Science;

    Sammanfattning : Dioxygenase-cytochrome P450 (DOX-CYP) fusion enzymes are present in diverse human and plant pathogenic fungi. They oxygenate fatty acids to lipid mediators which have regula­tory functions in fungal development and toxin production. LÄS MER

  3. 7. Nucleotide Binding to Trimeric dUTPase: Studies on Recombinant Enzymes using Kinetic and Spectroscopic Methods

    Författare :Johan Nord; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; C-terminus; dUTPase; EIAV; Escherichia coli; flexibility; Homo sapiens; kinetics; NMR; nucleotide metabolism; rate constants of lig-and binding; production of recombinant enzyme; dUTP; specificity; Biochemistry; Metabolism; Biokemi;

    Sammanfattning : Recombinant dUTPase from the bacterium Escherichia coli, the retrovirus equine infectious anemia virus (EIAV) and Homo sapiens have been made available for physico-chemical characterisation. The protocols developed have also proven to be suitable for production of enzymes modified by isotope labelling or other methods. LÄS MER

  4. 8. Combinatorial protein engineering applied to enzyme catalysis and molecular recognition

    Författare :Malin Eklund; KTH; []
    Nyckelord :phage display; selection; enzyme; affinity domains; crystal structure; affibody; lipase; protein engineering; protein A; assembly;

    Sammanfattning : The recent development of methods for constructing andhandling large collections (libraries) of proteins, from whichvariants with desired traits can be isolated, hasrevolutionized the field of protein engineering. Key elementsof such methods are the various ways in which the genotypes(the genes) and the phenotypes (the encoded proteins) arephysically linked during the process. LÄS MER

  5. 9. Physiological Engineering of Xylose Utilisation by Recombinant Saccharomyces cerevisiae

    Författare :Nina Quednau Meinander; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; protein burden; physiology; fed-batch fermentation; chemostat; recombinant Saccharomyces cerevisiae; ethanol; xylose; xylitol; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

    Sammanfattning : Xylitol production by recombinant, XYL1-expressing Saccharomyces cerevisiae was investigated in fed-batch fermentation using different cosubstrates for growth, and generation of reduced cofactors and maintenance energy. Xylose was converted into xylitol with 1:1 yield. LÄS MER

  7. 10. Bioprocess Monitoring and Control in Pseudomonas cepacia and recombinant Escherichia coli cultivations

    Författare :Anita Tocaj; LTH Ingenjörshögskolan vid Campus Helsingborg; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; protein L; expanded bed; flow-injection analysis; recombinant protein production; acetic acid; on-line measurements; Pseudomonas cepacia; recombinant Escherichia coli; Fed-batch cultivations; control strategies; xylanase deletion derivatives from Rhodothermus marinus; Biotechnology; Bioteknik;

    Sammanfattning : The control of cultivation conditions and its influence on growth and production have been investigated. The use of fed-batch processes have been demonstrated for several cultivation systems and so has the use of different control signals in these systems. LÄS MER