Sökning: "production of recombinant enzyme"

Visar resultat 21 - 25 av 68 avhandlingar innehållade orden production of recombinant enzyme.

1. 21. Production of Perdeuterated Proteins in Escherichia coli for Structural Studies by Neutron Crystallography

   Författare :Vinardas Kelpsas; Molekylär cellbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Perdeuteration; Escherichia coli; triose-phosphate isomerase; Neutron crystallography; crystals;

   Sammanfattning : Neutron macromolecular crystallography (NMX) is a complementary technique to X-ray crystallography leveraging neutron scattering properties of the hydrogen isotope deuterium (2H). Production of deuterium labelled molecules together with large crystal requirements for neutron crystallography are few of several bottlenecks limiting implementation of this technique for a wider range of proteins. LÄS MER

3. 22. Sesquiterpene synthases in Artemisia annua: Cloning, Expression, and Characterisation of the Recombinant Enzymes

   Författare :Per Mercke; Kemiska institutionen; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Artemisia annua; Sesquiterpene synthases; artemisinin; Plant biochemistry; Växtbiokemi;

   Sammanfattning : Plants produce a multitude of different compounds that may have biotic effects. Artemisia annua, produces a compound used as a drug against multi-drug-resistant strains of Plasmodium falciparum, the parasite causing malaria. LÄS MER

4. 23. Oxidation of Ketones: A (Chemo-) Enzymatic Approach Using Oxygenases and Hydrolases

   Författare :Georgina Chávez; Bioteknik; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; cross linked enzyme aggregates.; caprolactone; perhydrolase; Lipase B from Candida antarctica;

   Sammanfattning : Oxidation reactions are important in organic chemistry as well as in nature. In industry, oxidations are commonly used for the synthesis of chemicals and pharmaceuticals, however such processes have a number of limitations, they use chlorinated solvents, stoichiometric oxidation reagents, and in some cases the reagents that have risks of explosion during transportation and storage. LÄS MER

5. 24. Expanded knowledge on silk assembly for development of bioactive silk coatings

   Författare :Linnea Nilebäck; My Hedhammar; Renko de Vries; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Recombinant spider silk; Self-assembly; Biomaterial; Implant coating; Functionalization; Multi-functional; Bioactive; Cell culture scaffold; Antibacterial; Bioteknologi; Biotechnology;

   Sammanfattning : Silk is a fascinating natural material made from proteins that self-assemble through structural rearrangements into one of the toughest materials known. As silk is protein-based, durable and elastic, it has many features that makes it suitable as a scaffold material for tissue engineering. LÄS MER

7. 25. Microbial and enzymatic syntheses of polymer building blocks through selective transformations of polyols and furans

   Författare :Mahmoud Sayed Ali Sayed; Bioteknik; []
   Nyckelord :Mycobacterium sp. MS1601; G. oxydans; oxidase; lipase; trimethylolpropane TMP ; 2; 2- bis hydroxymethyl butyric acid BHMB ; 5-hydroxymethyl furfural 5-HMF ; 5-hydroxymethyl-2-furan carboxylic acid HMFCA ; 2; 5-furandicarboxylic acid FDCA ; cyclic carbonate ; estrification; Biotransformation; polymer building blocks; product recovery; selective oxidation; bioprocess engneering; cell recycling; cell bleeding; DNA recombinant technology; in-silico analysis;

   Sammanfattning : AbstractTransition from fossil- to bio-based economy is a critical step towards reduction of greenhouse gas emissions and climate change, and hence for achievement of sustainable communities and environment. In order to be fossil-free, the chemical and material industry is in need of carbon-neutral building blocks from renewable resources for the diverse array of products that are currently produced from olefins and aromatics. LÄS MER