Sökning: "recombinant Escherichia coli"

Visar resultat 1 - 5 av 81 avhandlingar innehållade orden recombinant Escherichia coli.

1. 1. Membrane and secretory protein production in Escherichia coli

   Författare :Alexandros Karyolaimos; Jan-Willem de Gier; Matthew DeLisa; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; recombinant protein; tunable protein production; cytoplasmic membrane; periplasm; signal peptide; force profile analysis; co-translational folding; Biochemistry; biokemi;

   Sammanfattning : Escherichia coli (E. coli) is the most widely used bacterium for the production of recombinant proteins. However, the production of proteins in its cytoplasmic membrane or periplasm is challenging. Therefore, the aim of this doctoral thesis was to develop setups for enhancing the production of membrane and secretory proteins in E. LÄS MER

3. 2. Impact of glucose uptake rate on recombinant protein production in Escherichia coli

   Författare :Emma Bäcklund; Gen Larsson; Olle Holst; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; AIDA-autotransporter; Escherichia coli; fed-batch; glucose uptake rate; integral membrane proteins; outer membrane proteins; periplasmic retention; phosphotransferase system; recombinant proteins; specific growth rate; surface expression; Biology; Biologi;

   Sammanfattning : Escherichia coli (E. coli) is an attractive host for production of recombinant proteins, since it generally provides a rapid and economical means to achieve high product quantities. LÄS MER

4. 3. Control of Proteolysis of Recombinant Proteins in Escherichia coli

   Författare :Aleksei Rozkov; KTH; []
   Nyckelord :proteolysis; recombinant protein; E. coli; bioprocess scale-up; protein A; ATP; sulfite; amino acids; growth inhibition; fed-batch culture; ZZT2;

   Sammanfattning : .... LÄS MER

5. 4. Bioprocess Monitoring and Control in Pseudomonas cepacia and recombinant Escherichia coli cultivations

   Författare :Anita Tocaj; LTH Ingenjörshögskolan vid Campus Helsingborg; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; protein L; expanded bed; flow-injection analysis; recombinant protein production; acetic acid; on-line measurements; Pseudomonas cepacia; recombinant Escherichia coli; Fed-batch cultivations; control strategies; xylanase deletion derivatives from Rhodothermus marinus; Biotechnology; Bioteknik;

   Sammanfattning : The control of cultivation conditions and its influence on growth and production have been investigated. The use of fed-batch processes have been demonstrated for several cultivation systems and so has the use of different control signals in these systems. LÄS MER

7. 5. Methodology for high-throughput production of soluble recombinant proteins in Escherichia coli

   Författare :Katrin Markland; Gen Larsson; Pär Nordlund; Jenny Ottosson; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; high-throughput methodology; recombinant protein production; solubility; productivity; quality; cultivation technology; parallel reactors; Biochemistry; Biokemi;

   Sammanfattning : The aim of this work was to investigate and determine central parameters that can be used to control and increase the solubility, quality and productivity of recombinant proteins. These central parameters should be applicable under the constraints of high-throughput protein production in Escherichia coli. LÄS MER