Sökning: "production of recombinant enzyme"

Visar resultat 11 - 15 av 68 avhandlingar innehållade orden production of recombinant enzyme.

1. 11. RECOMBINANT PEROXIDASES AND XYLANASES: I. Cloning and production of a peroxidase from horseradish II. Characterisation of functional domains of thermostable xylanases from Rhodothermus marinus

   Författare :Eva Bartonek Roxå; Bioteknik; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; amorphous cellulose; xylan; CBD; carbohydrate binding domains; insect cells; Baculovirus; catalytically active; recombinant horseradish peroxidase; HRP-n; PASC; beta-glucan; Biotechnology; Bioteknik;

   Sammanfattning : Part I: Plant peroxidases are useful in many areas of application e.g. as tracer enzymes for clinical applications and in biosensors as well as for industrial purposes like in waste water treatment and bleaching processes. A gene encoding a new, neutral horseradish peroxidase isoenzyme, HRP-n, was isolated. LÄS MER

3. 12. Ethanol production by recombinant and natural xylose-utilising yeasts

   Författare :Anna Eliasson; Teknisk mikrobiologi; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; lignocellulose; electron transport; redox balance; Pichia stipitis; Saccharomyces cerevisiae; ethanol; xylose; physiology; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

   Sammanfattning : The xylose-fermenting capacity of recombinant Saccharomyces cerevisiae carrying XYL1 and XYL2 from Pichia stipitis, which encode xylose reductase (XR) and xylitol dehydrogenase (XDH), respectively, is poor due to high xylitol formation. Whereas, P. LÄS MER

4. 13. Structural Studies on PP2A and Methods in Protein Production

   Författare :Auður Magnúsdóttir; Pär Nordlund; Herman Van Tilbeurgh; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; PP2A; PTPA; B56γ; Protein Production; Structural biology; Strukturbiologi; biokemi; Biochemistry;

   Sammanfattning : PP2A is a major phosphatase in the cell that participates in multiple cell signaling pathways. It is a heterotrimer of a core dimer and variable regulatory subunits. Details of its structure, function and regulation are slowly emerging. Here, the structure of two regulators of PP2A are de-scribed; PTPA and B56γ. LÄS MER

5. 14. Application of Synthetic Biology for Biopolymer Production using Saccharomyces cerevisiae

   Författare :Alejandro Muñoz De Las Heras; Teknisk mikrobiologi; []
   Nyckelord :Saccharomyce cerevisiae; Xylose; Poly-3-d-hydroxybutyrate PHB ; NADH; NADPH; Acetyl-CoA; Metabolic engineering;

   Sammanfattning : Plastics are versatile, cheap and durable materials that are omnipresent in modern society. Since most of them are derived from crude oil and are not biodegradable, their production leads to the depletion of fossil fuels and the accumulation of enormous amounts of plastic waste that pollutes ecosystems worldwide. LÄS MER

7. 15. Recombinant Enzymes in Pyrosequencing Technology

   Författare :Nader Nourizad; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; apyrase; pyrosequencing; pichia pastoris; protein expression; fusion protein; luciferase; DNA template; clone checking; DNA polymerase;

   Sammanfattning : Pyrosequencing is a DNA sequencing method based on thedetection of released pyrophosphate (PPi) during DNA synthesis.In a cascade of enzymatic reactions, visible light isgenerated, which is proportional to the number of nucleotidesincorporated into the DNA template. When dNTP(s) areincorporated into the DNA template, inorganic PPi is released. LÄS MER