Sökning: "ligand binding"

Visar resultat 16 - 20 av 564 avhandlingar innehållade orden ligand binding.

1. 16. DNA Interactions with Chiral Polyaza-aromatic Ruthenium(II) Complexes

   Författare :Per Lincoln; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; phenanthroline; binding geometry; binding isotherm; DNA; dppz; linear dichroism; intercalation; ruthenium; cooperative binding;

   Sammanfattning : The binding to DNA of a series of chiral ruthenium complexes is studied with optical spectroscopy techniques, including isotropic absorption, linear dichroism (LD) and emission anisotropy as well as steady-state and time-resolved luminescence. The thesis focuses on the ìlight-switchî complex [Ru(phen)2dppz]2+ which binds to DNA by intercalation and luminesces when bound to DNA but not in pure buffer solution (phen=1,10-phenanthroline; dppz=dipyrido[3,2-a:2',3'-c]phenazine). LÄS MER

3. 17. Multivariate design of molecular docking experiments : An investigation of protein-ligand interactions

   Författare :David Andersson; Anna Linusson Jonsson; Gabriele Cruciani; Umeå universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Molecular docking; chemometrics; multivariate analysis; principal component analysis; PCA; design of experiments; DoE; partial least-square projections to latent structures; PLS; scoring functions; ligand-binding cavity; major histocompatibility complex; MHC; glycopeptide; T-cell.; Pharmaceutical chemistry; Läkemedelskemi; datorlingvistik; computational linguistics;

   Sammanfattning : To be able to make informed descicions regarding the research of new drug molecules (ligands), it is crucial to have access to information regarding the chemical interaction between the drug and its biological target (protein). Computer-based methods have a given role in drug research today and, by using methods such as molecular docking, it is possible to investigate the way in which ligands and proteins interact. LÄS MER

4. 18. Deciphering Binding Patterns of Therapeutic Antibodies with Immune Cells : From Method Development to Application

   Författare :Sina Bondza; Jos Buijs; Marika Nestor; Mark Cragg; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; affinity; binding kinetics; CD20; cell-based assay; immunology; receptor-ligand interaction; therapeutic antibody; Immunologi; Immunology; Medical Cell Biology; Medicinsk cellbiologi;

   Sammanfattning : Reversible binding, for example between signaling molecules and receptors on the cell surface, is one of the main means to communicate information in cellular systems. Knowledge about how molecules interact is crucial for both understanding biological function and for therapeutic intervention. LÄS MER

5. 19. Surface Properties of Antibodies and their Complexes with Antigens Studied by LLPC

   Författare :Christer Wingren; Institutionen för immunteknologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; ligand-binding.; surface properties; isomerism; conformational changes; antibody; specificity; two-phase partitioning; antigen-binding sites; Biochemistry; Metabolism; Biokemi; metabolism;

   Sammanfattning : The main objective of this thesis was to gain further insight into the relationship between the exposed surfaces and the functional properties of antibodies and their complexes with antigens in solution. To study this relationship, a new technique, liquid-liquid partition chromatography (LLPC) in aqueous two-phase systems, was developed further with respect to sensitivity, selectivity and reproducibility. LÄS MER

7. 20. Computational Analysis of Molecular Recognition Involving the Ribosome and a Voltage Gated K+ Channel

   Författare :Martin Andér; Johan Åqvist; Lennart Nilsson; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; computer simulations; molecular dynamics; ligand binding; binding free energy; linear interaction energy; codon recognition; translation termination; release factor; voltage gated potassium ion channel; Kv1.5; Structural biology; Strukturbiologi; Biochemistry; Biokemi; Biokemi; Biochemistry;

   Sammanfattning : Over the last few decades, computer simulation techniques have been established as an essential tool for understanding biochemical processes. This thesis deals mainly with the application of free energy calculations to ribosomal complexes and a cardiac ion channel. LÄS MER