Sökning: "immunogold electron microscopy."

Visar resultat 1 - 5 av 14 avhandlingar innehållade orden immunogold electron microscopy..

1. 1. Development and application of advanced electron microscopy techniques for materials in biological systems

   Författare :Pernilla Nevsten; Centrum för analys och syntes; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; multiple-unit drug; EFTEM; EELS; cyanine dye; SSAT; cryo-TEM; virus; TEM; apoptosis; SEM; AFm; α-TCP; apatite; XEDS; TEM grid; microscopy supports;

   Sammanfattning : Theoretically, electron microscopy and scanning probe microscopy offer information on a scale beyond the optical microscope. However, the quality of the micrograph and the obtained information is limited by the sample preparation. LÄS MER

3. 2. Optimizing Enzyme Immobilization in Mesoporous Silica - A Spectroscopic Study of the Dynamics and Spatial Distribution of the Confined Enzymes

   Författare :Pegah Sadat Nabavi Zadeh; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; fluorescence spectroscopy; dynamics; mesoporous silica; Immunogold staining; Transmission electron microscopy; anisotropy; microviscosity; real-time monitoring; Cy5.; Förster resonance energy transfer; Enzyme immobilization; Cy3;

   Sammanfattning : Enzymes as biological catalysts are immobilized in porous materials to improve the enzyme activity and simplify their purification from the reaction media in biocatalytic applications. Mesoporous silica (MPS) particles are used as a promising support material for the immobilization of enzymes. LÄS MER

4. 3. Molecular mechanisms of synaptic transmission and plasticity in spinal sensory pathways

   Författare :Max Larsson; Neurofysiologi; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; neurophysiology; neuropsychology; Neurology; electron microscopy; synaptic plasticity; synapse; pain; spinal cord; hyperalgesia; Neurologi; neuropsykologi; neurofysiologi;

   Sammanfattning : An intense, potentially tissue-damaging (noxious) stimulus may lead to enhanced pain sensation of subsequent noxious stimuli (hyperalgesia) and to the perceiving of innocuous stimuli as painful (allodynia). This is thought to result to a large extent from strengthening of synapses established by primary afferent fibers onto spinal cord dorsal horn neurons. LÄS MER

5. 4. Evaluation of UHT milk processed by direct steam injection and steam infusion technology

   Författare :Bozena Malmgren; Livsmedelsteknik; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; sedimentation; gel formation; microstructure; TEM transmission electron microscopy; immunogold labelling; heat effects; Technological sciences; Teknik; Food and drink technology; Livsmedelsteknik; proteolysis; steam infusion; UHT milk; steam injection;

   Sammanfattning : UHT direct steam injection and steam infusion are widely used; however there is no comparison of their impact on milk components. This study evaluates the structural changes at different steps during the UHT processing of whole milk in a full-scale UHT plant by transmission electron microscopy (TEM) and particle size measurements, and follows the enzymatic activity. LÄS MER

7. 5. Coordination of endocytosis at the synaptic periactive zone

   Författare :Emma Evergren; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :Synapse; endocytosis; clathrin; dynamin; amphiphysin; synapsin; intersectin; actin cytoskeleton; immunogold electron microscopy.;

   Sammanfattning : Neurons in the central nervous system communicate via specialized junctions called synapses. Neurotransmitter-filled vesicles are clustered at these junctions, where they are released in response to synaptic activity. To sustain reliable neurotransmission the synaptic vesicles are recycled locally. LÄS MER