Sökning: "Cy5."
Hittade 4 avhandlingar innehållade ordet Cy5..
1. Optimizing Enzyme Immobilization in Mesoporous Silica - A Spectroscopic Study of the Dynamics and Spatial Distribution of the Confined Enzymes
Sammanfattning : Enzymes as biological catalysts are immobilized in porous materials to improve the enzyme activity and simplify their purification from the reaction media in biocatalytic applications. Mesoporous silica (MPS) particles are used as a promising support material for the immobilization of enzymes. LÄS MER
2. Advanced fluorescence-based fluctuation methods for biosensing
Sammanfattning : In addition to use traditional fluorescence parameters for biological studies,the main focus of this thesis is on the monitoring of photoinduced fluorophoretransient states. These dark states, such as the triplet state, redox states andphotoisomers that are formed upon excitation, are long-lived in comparisonto the fluorescent singlet state and therefor more sensitive to the surroundingenvironment and their sensitivity are to biological relevant conditions. LÄS MER
3. Mimicking the action of ribonucleases : studies on RNase A and design of PNA based artificial enzymes
Sammanfattning : A 3’-deoxy-3’-C-methylenephosphonate modified diribonucleotide is highly resistant to degradation by spleen phosphodiesterase and not cleaved at all by snake venom phosphodiesterase. Despite that both the vicinal 2-hydroxy nucleophile and the 5’-oxyanion leaving group are intact, the 3’-methylenephosponate RNA modification is also highly resistant towards the action of RNase A. LÄS MER
4. Custom-tailored DNA origami mechanics for cellular applications
Sammanfattning : DNA molecules have been used as the building block for the self-assembly of artificial nanostructures. In particular, the DNA origami method has made the design of DNA nanostructures more robust and approachable. LÄS MER