Sökning: "Förster resonance energy transfer"

Visar resultat 1 - 5 av 18 avhandlingar innehållade orden Förster resonance energy transfer.

  1. 1. Förster Resonance Energy Transfer - from single molecule spectroscopy to imaging

    Författare :Evangelos Sisamakis; Jerker Widengren; Paul R Selvin; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; FRET; FCS; single-molecule biophysics;

    Sammanfattning : During the last fifteen years several methods have been developed for probing biomolecules (DNA, RNA, proteins) one at a time. Among these methods fluorescence spectroscopy and in particular its many implementations for monitoring Förster Resonance Energy Transfer (FRET), have attracted much interest. LÄS MER

  3. 2. Transient State Fluorescence Microscopy - method development and biological applications : Exploiting the dark states of fluorophores to measure oxygen concentrations, redox state, Förster resonance energy transfer and membrane viscosity

    Författare :Thiemo Spielmann; Jerker Widengren; Karsten König; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Transient States imaging TRAST ; Triplet State imaging; fluorescence microscopy; modulated excitation; triplet state; radical state; trans-cis isomerisation;

    Sammanfattning : Due to their long lifetime, triplet, redox and other transient states of fluorophores are highly sensitive to the micro-environment. Imaging their spatial distribution in biological samples can therefore help answer interesting questions about the metabolism, molecular interactions and dynamics in living cells. LÄS MER

  4. 3. Study of GaN Based Nanostructures and Hybrids

    Författare :Mathias Forsberg; Galia Pozina; Ching-Lien Hsiao; Kevin P. O'Donnell; Linköpings universitet; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Sputtering; Forster; Semiconductor; Nitride; GaN; Hybrid; Nanorods; Nanostructure; Thin film; Förstoffning; Sputtring; Förster; Halvledare; Nitrid; GaN; Hybrider; Nanostavar; Nanostrukturer; tunnfilmer;

    Sammanfattning : GaN and its alloys with Al and In belong to the group III nitride semiconductors and are today the materials of choice for efficient white light emitting diodes (LEDs) enabling energy saving solid state lighting. Currently, there is a great interest in the development of novel inexpensive techniques to fabricate hybrid LEDs combining high quality III-N quantum well (QW) structures with inexpensive colloidal nanoparticles or conjugated polymers. LÄS MER

  5. 4. Optimizing Enzyme Immobilization in Mesoporous Silica - A Spectroscopic Study of the Dynamics and Spatial Distribution of the Confined Enzymes

    Författare :Pegah Sadat Nabavi Zadeh; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; fluorescence spectroscopy; dynamics; mesoporous silica; Immunogold staining; Transmission electron microscopy; anisotropy; microviscosity; real-time monitoring; Cy5.; Förster resonance energy transfer; Enzyme immobilization; Cy3;

    Sammanfattning : Enzymes as biological catalysts are immobilized in porous materials to improve the enzyme activity and simplify their purification from the reaction media in biocatalytic applications. Mesoporous silica (MPS) particles are used as a promising support material for the immobilization of enzymes. LÄS MER

  7. 5. Advanced Fluorescence Microscopy to Study Plasma Membrane Protein Dynamics

    Författare :Joachim Piguet; Horst Vogel; Ruud Hovius; Jerker Widengren; Antoine Triller; Switzerland Ecole Polytechnique Fédérale de Lausanne (EPFL); []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; fluorescence; microscopy; plasma membrane; membrane proteins; membrane proteins diffusion; single-molecule imaging; single-molecule tracking; pentameric ligand-gated ion channels; nicotinic acetylcholine receptor; serotonin receptor; synapse; neuromuscular junction; post-synaptic scaffold; rapsyn; myopathies; fluorescent proteins; Förster resonance energy transfer; FRET imaging; protein-protein interactions; protein trafficking; photo-activatable proteins; Biological Physics; Biologisk fysik;

    Sammanfattning : Membrane protein dynamics is of great importance for living organisms. The precise localization of proteins composing a synapse on the membrane facing a nerve terminus is essential for proper functioning of the nervous system. In muscle fibers, the nicotinic acetylcholine is densely packed under the motor nerve termini. LÄS MER