Sökning: "activity assay"

Visar resultat 1 - 5 av 498 avhandlingar innehållade orden activity assay.

  1. 1. HIV-1 reverse transcriptase activity assays based on monoclonal antibodies to native enzyme : Applications on studies of antiviral substances and immunological variation of reverse transcriptase

    Författare :Ann-Sofie Rytting; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; HIV-1; reverse transcriptase; monoclonal antibody; activity assay; subtype; RT inhibitor; Genetik; Clinical genetics; Klinisk genetik; Medical Genetics; medicinsk genetik;

    Sammanfattning : Specific detection of HIV-1 reverse transcriptase (RT) activity in crude materials was enhanced using a capture assay for immunopurification and subsequent activity assay. The purification step was achieved by anti-HIV-1 RT monoclonal antibodies (Mabs) immobilized onto a solid carrier. LÄS MER

  3. 2. Plasminogen Activator Inhibitor 1 in Platelets - Studies of Synthesis, Activity, and Glycosylation Patterns

    Författare :Helén Brogren; Göteborgs universitet; []
    Nyckelord :PAI-1; platelets; plasma; fibrinolysis; synthesis; polymorphism; glycosylation; activity; platelet mRNA;

    Sammanfattning : Plasminogen activator inhibitor 1 (PAI-1) is the main physiological inhibitor of tissue-type plasminogen activator. Thus PAI-1 plays an important role in decreasing the fibrinolytic activity in human blood. PAI-1 is present in high concentrations in platelets and also in low concentrations in plasma, but the source of plasma PAI-1 is not known. LÄS MER

  4. 3. Haemophilia B - Diagnostic Insights, Genetic Aspects and Clinical Outcomes

    Författare :Kristina Kihlberg; Institutionen för translationell medicin; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Coagulation factor IX; Haemophilia B; Haemophilia A; F9 variant; Assay discrepancy; Chromogenic assay; One-stage assay; Bleeding disorders; Quality of life; Immune tolerance induction; Non-neutralising antibodies; Inhibitors; Treatment Adherence; Arthropathy;

    Sammanfattning : Haemophilia B (HB) is a rare inherited bleeding disorder caused by the deficiency of coagulation factor IX (FIX). The major clinical issues are bleedings, often targeting the joints, and the development of neutralising antibodies, i.e. inhibitors, to the FIX replacement therapy. LÄS MER

  5. 4. Reverse Transcriptase Activity Assays for Retrovirus Quantitation and Characterization

    Författare :Anders Malmsten; Ulf Gyllensten; Clas Källander; Lynn Morris; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Medicine; retrovirus; reverse transcriptase; enzyme activity assay; MuLV; HIV; RT purification; viral load; drug susceptibility; Medicin; Dermatology and venerology; clinical genetics; internal medicine; Dermatologi och venerologi; klinisk genetik; invärtesmedicin;

    Sammanfattning : Reverse transcriptase (RT) is a crucial enzyme for retrovirus replication, and its presence in the virion is indispensable for infectivity. This thesis illustrates the use of RT activity assays as tools for quantitation and characterization of different retroviruses, particularly HIV. LÄS MER

  7. 5. Effect of surface characteristics on cellular adherence and activity

    Författare :Marjan Dorkhan; Malmö högskola; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Bacteria; microbial biofilm; dental implant; streptococci; oral bacteria; salivary pellicle; early colonizers; titanium oxide; microbial activity; oral keratinocytes; gingival fibroblasts; cell attachment; surface modification; anodic oxidation; anatase;

    Sammanfattning : Ersättning av förlorade tänder med tandimplantat är numera en vanlig behandlingsmetod med generellt goda resultat. Antalet implantatsystem på den internationella marknaden har ökat kraftigt under senare år. LÄS MER