Sökning: "RT purification"
Visar resultat 1 - 5 av 12 avhandlingar innehållade orden RT purification.
1. Reverse Transcriptase Activity Assays for Retrovirus Quantitation and Characterization
Sammanfattning : Reverse transcriptase (RT) is a crucial enzyme for retrovirus replication, and its presence in the virion is indispensable for infectivity. This thesis illustrates the use of RT activity assays as tools for quantitation and characterization of different retroviruses, particularly HIV. LÄS MER
2. HIV-1 reverse transcriptase activity assays based on monoclonal antibodies to native enzyme : Applications on studies of antiviral substances and immunological variation of reverse transcriptase
Sammanfattning : Specific detection of HIV-1 reverse transcriptase (RT) activity in crude materials was enhanced using a capture assay for immunopurification and subsequent activity assay. The purification step was achieved by anti-HIV-1 RT monoclonal antibodies (Mabs) immobilized onto a solid carrier. LÄS MER
3. Reverse transcriptase assay for analysis of resistance to anti-HIV drugs and their mechanism of action
Sammanfattning : Reverse transcriptase (RT) is a viral enzyme and one of the main targets for drugs against human immunodeficiency virus (HIV). This work presents a set of sensitive non-radioactive RT assays were developed: standard RT inhibition assay (IC assay); RT binding inhibition assay; RTprotein detection assay and template-primer destruction assay or chain termination assay (CT assay). LÄS MER
4. Human immunodeficiency virus type 1 proteinase : regulation of function and catalytic activity
Sammanfattning : HIV-l proteinase activity is a prerequisite for viral replication, and one of the main targets for anti-HlV therapy. Although potent proteinase inhibitors are currently being approved for treatment of AIDS patients, these drugs are unlikely to provide treatment which can give patients a life of normal quality and duration. LÄS MER
5. Structure-aided design of antiviral drugs : Application of the method on HIV-1 protease and SIV reverse transcriptase
Sammanfattning : Many efforts have been made to control the AIDS epidemic. Extensive studies have been done on the biology, biochemistry, and structural biology of HIV in the search for antiviral drugs. The viral-encoded enzymes reverse transcriptase and protease have been main targets for drug design. LÄS MER