Sökning: "enzyme activity assay"

Visar resultat 1 - 5 av 153 avhandlingar innehållade orden enzyme activity assay.

  1. 1. Amine Transaminases in Biocatalytic Amine Synthesis

    Författare :Henrik Land; Per Berglund; Helen C. Hailes; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Amine Transaminase; Biocatalysis; Transamination; Reductive Amination; Enzyme; Enzyme Engineering; Equilibrium Displacement; Screening; Enzyme Stability; Bioteknologi; Biotechnology;

    Sammanfattning : The use of enzymes, nature´s own catalysts, both isolated or as whole cells to perform chemical transformations is called biocatalysis. As a complement to classical chemical catalysis, biocatalysis can be an environmentally friendly and more economical option in the production and synthesis of chemicals. LÄS MER

  3. 2. HIV-1 reverse transcriptase activity assays based on monoclonal antibodies to native enzyme : Applications on studies of antiviral substances and immunological variation of reverse transcriptase

    Författare :Ann-Sofie Rytting; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; HIV-1; reverse transcriptase; monoclonal antibody; activity assay; subtype; RT inhibitor; Genetik; Clinical genetics; Klinisk genetik; Medical Genetics; medicinsk genetik;

    Sammanfattning : Specific detection of HIV-1 reverse transcriptase (RT) activity in crude materials was enhanced using a capture assay for immunopurification and subsequent activity assay. The purification step was achieved by anti-HIV-1 RT monoclonal antibodies (Mabs) immobilized onto a solid carrier. LÄS MER

  4. 3. Reverse Transcriptase Activity Assays for Retrovirus Quantitation and Characterization

    Författare :Anders Malmsten; Ulf Gyllensten; Clas Källander; Lynn Morris; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Medicine; retrovirus; reverse transcriptase; enzyme activity assay; MuLV; HIV; RT purification; viral load; drug susceptibility; Medicin; Dermatology and venerology; clinical genetics; internal medicine; Dermatologi och venerologi; klinisk genetik; invärtesmedicin;

    Sammanfattning : Reverse transcriptase (RT) is a crucial enzyme for retrovirus replication, and its presence in the virion is indispensable for infectivity. This thesis illustrates the use of RT activity assays as tools for quantitation and characterization of different retroviruses, particularly HIV. LÄS MER

  5. 4. Optimizing Enzyme Immobilization in Mesoporous Silica - A Spectroscopic Study of the Dynamics and Spatial Distribution of the Confined Enzymes

    Författare :Pegah Sadat Nabavi Zadeh; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; fluorescence spectroscopy; dynamics; mesoporous silica; Immunogold staining; Transmission electron microscopy; anisotropy; microviscosity; real-time monitoring; Cy5.; Förster resonance energy transfer; Enzyme immobilization; Cy3;

    Sammanfattning : Enzymes as biological catalysts are immobilized in porous materials to improve the enzyme activity and simplify their purification from the reaction media in biocatalytic applications. Mesoporous silica (MPS) particles are used as a promising support material for the immobilization of enzymes. LÄS MER

  7. 5. Haemophilia B - Diagnostic Insights, Genetic Aspects and Clinical Outcomes

    Författare :Kristina Kihlberg; Institutionen för translationell medicin; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Coagulation factor IX; Haemophilia B; Haemophilia A; F9 variant; Assay discrepancy; Chromogenic assay; One-stage assay; Bleeding disorders; Quality of life; Immune tolerance induction; Non-neutralising antibodies; Inhibitors; Treatment Adherence; Arthropathy;

    Sammanfattning : Haemophilia B (HB) is a rare inherited bleeding disorder caused by the deficiency of coagulation factor IX (FIX). The major clinical issues are bleedings, often targeting the joints, and the development of neutralising antibodies, i.e. inhibitors, to the FIX replacement therapy. LÄS MER