Sökning: "High-Throughput Nucleotide Sequencing"

Visar resultat 1 - 5 av 19 avhandlingar innehållade orden High-Throughput Nucleotide Sequencing.

1. 1. Exploring genetic heterogeneity in cancer using high-throughput DNA and RNA sequencing

   Författare :Erik Fasterius; Cristina Al-Khalili Szigyarto; Mathias Uhlén; Peter-Bram ’t Hoen; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; Biotechnology; bioinformatics; RNA-seq; WGS; WES; systems biology; variant analysis; single nucleotide variant; gene expression; machine learning; clustering; open source; R; bioconductor; Python; Bioteknologi; Biotechnology;

   Sammanfattning : High-throughput sequencing (HTS) technology has revolutionised the biomedical sciences, where it is used to analyse the genetic makeup and gene expression patterns of both primary patient tissue samples and models cultivated in vitro. This makes it especially useful for research on cancer, a disease that is characterised by its deadliness and genetic heterogeneity. LÄS MER

3. 2. Statistical assessment of genomic variability in tumours and bacterial communities

   Författare :Anna Rehammar; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; somatic mutations; hierarchical Bayesian modelling; cancer genetics; high-throughput sequencing; metagenomics; personalised diagnostics;

   Sammanfattning : Current high-throughput DNA sequencing technologies have the ability to generate large amounts of high-resolution genomic data. The high dimensionality in combination with the substantial levels of technical errors and biological variability typically present in the data make, however, the analysis challenging. LÄS MER

4. 3. Diversity of Skin Infections

   Författare :Johanna Ekström; Malmö Klinisk mikrobiologi; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Human papillomavirus; high-throughput sequencing; Staphylococcus auereus; multiple displacement amplification; non melanoma skin cancer;

   Sammanfattning : The identification of infectious agents in cancer has been one of the most rewarding endeavors in cancer research. Currently about 20% of the global cancer burden is linked to an infection. A common characteristic of virus-induced cancer is an increased incidence in immunosuppressed patients, presumably because of impaired host control of virus. LÄS MER

5. 4. Metagenomic Characterization of the Gut Microbiome in Cohorts of Elderly

   Författare :Sukithar K Rajan; Dirk Repsilber; Carl Mårten Lindqvist; Robert Jan Brummer; Lars-Gustav Snipen; Örebro universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Gut microbiota; metagenomics; High-Throughput Nucleotide Sequencing; reference sequence database; methods validation; microbial function; host-microbe interaction;

   Sammanfattning : Human gut microbiota plays a vital role in maintaining host health. This thesis aims to investigate the gut microbial population and function using next generation sequencing (NGS) data from faecal samples. Paper I examines the influence of sequencing depth and analysis methods in microbiota profiling using NGS whole genome sequencing (WGS) data. LÄS MER

7. 5. Molecular Tools for Nucleic Acid Analysis

   Författare :Deirdre O'Meara; KTH; []
   Nyckelord :nucleic acid capture; modular probes; biosensor; bio-magnetic separation; hepatitis C; sequencing; pyrosequencing; mutation detection; HIV-1; drug resistance; SNP; allele-specific extension; apyrase; genotyping.;

   Sammanfattning : Nucleic acid technology has assumed an essential role invarious areas ofin vitrodiagnostics ranging from infectious diseasediagnosis to human genetics. An important requirement of suchmolecular methods is that they achieve high sensitivity andspecificity with a fast turnaround time in a cost-effectivemanner. LÄS MER