Sökning: "pyrosequencing"
Visar resultat 1 - 5 av 106 avhandlingar innehållade ordet pyrosequencing.
1. Pyrosequencing
Sammanfattning : Pyrosequencing is a method that can be used in almost allDNA sequencing applications. The method uses coupled reactionsof ATP sulfurylase and firefly luciferase to monitor theinorganic pyrophosphate (PPi) released during nucleotideincorporation onto a DNA primer. LÄS MER
2. Pyrosequencing : automation, optimization and template preparation
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3. Pyrosequencing
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4. Recombinant Enzymes in Pyrosequencing Technology
Sammanfattning : Pyrosequencing is a DNA sequencing method based on thedetection of released pyrophosphate (PPi) during DNA synthesis.In a cascade of enzymatic reactions, visible light isgenerated, which is proportional to the number of nucleotidesincorporated into the DNA template. When dNTP(s) areincorporated into the DNA template, inorganic PPi is released. LÄS MER
5. Sensitive Forensic DNA Analysis : Application of Pyrosequencing and Real-time PCR Quantification
Sammanfattning : The field of forensic genetics is growing fast and the development and optimisation of more sensitive, faster and more discriminating forensic DNA analysis methods is highly important. In this thesis, an evaluation of the use of novel DNA technologies and the development of specific applications for use in forensic casework investigations are presented. LÄS MER