Sökning: "apyrase"

Visar resultat 1 - 5 av 15 avhandlingar innehållade ordet apyrase.

  1. 1. Cardioprotective treatment strategies

    Författare :Jesper vanderPals; Kardiologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ADC-1004; apyrase; Cardioprotection; ischemia reperfusion; hypothermia; t-PA;

    Sammanfattning : In myocardial ischemia-reperfusion (I/R) injury, complement activation, tissue plasminogen activator (t-PA) and extracellular adenosine triphosphate (ATP) release contribute to myocardial injury. ATP is degraded into adenosine by the enzyme apyrase, and adenosine possesses cardioprotective properties. LÄS MER

  2. 2. Recombinant Enzymes in Pyrosequencing Technology

    Författare :Nader Nourizad; KTH; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; apyrase; pyrosequencing; pichia pastoris; protein expression; fusion protein; luciferase; DNA template; clone checking; DNA polymerase;

    Sammanfattning : Pyrosequencing is a DNA sequencing method based on thedetection of released pyrophosphate (PPi) during DNA synthesis.In a cascade of enzymatic reactions, visible light isgenerated, which is proportional to the number of nucleotidesincorporated into the DNA template. When dNTP(s) areincorporated into the DNA template, inorganic PPi is released. LÄS MER

  3. 3. Molecular Tools for Nucleic Acid Analysis

    Författare :Deirdre O'Meara; KTH; []
    Nyckelord :nucleic acid capture; modular probes; biosensor; bio-magnetic separation; hepatitis C; sequencing; pyrosequencing; mutation detection; HIV-1; drug resistance; SNP; allele-specific extension; apyrase; genotyping.;

    Sammanfattning : Nucleic acid technology has assumed an essential role invarious areas ofin vitrodiagnostics ranging from infectious diseasediagnosis to human genetics. An important requirement of suchmolecular methods is that they achieve high sensitivity andspecificity with a fast turnaround time in a cost-effectivemanner. LÄS MER

  4. 4. Arrayed identification of DNA signatures

    Författare :Max Käller; Lundeberg Joakim; Mats Nilsson; KTH; []
    Nyckelord :ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; apyrase; allele-specific extension; competitive hybridization; DNA sequencing; genotyping; human papillomavirus HPV ; MC1R; microarray; mutation; p53; protease; Bioengineering; Bioteknik;

    Sammanfattning : In this thesis techniques are presented that aim to determine individual DNA signatures by controlled synthesis of nucleic acid multimers. Allele-specific extension reactions with an improved specificity were applied for several genomic purposes. Since DNA polymerases extend some mismatched 3’-end primers, an improved specificity is a concern. LÄS MER

  5. 5. Interactions between platelets and complement with implications for the regulation at surfaces

    Författare :Per H. Nilsson; Kristina Nilsson Ekdahl; John Lambris; Ian Nicholls; Berhane Ghebrehiwet; Linnéuniversitetet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; NATURVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; NATURAL SCIENCES; MEDICAL AND HEALTH SCIENCES; MEDICAL AND HEALTH SCIENCES; complement system activation; regulation ; platelets activation; regulation ; biomaterials; biocompatibility; chondroitin sulfate-A; apyrase; C1q; C3; factor H; C4BP; ADP; Biomedicinsk vetenskap; Biomedical Sciences;

    Sammanfattning : Disturbances of host integrity have the potential to evoke activation of innate immunologic and hemostatic protection mechanisms in blood. Irrespective of whether the activating stimulus is typically immunogenic or thrombotic, it will generally affect both the complement system and platelets to a certain degree. LÄS MER