Sökning: "multiple displacement amplification"

Visar resultat 1 - 5 av 12 avhandlingar innehållade orden multiple displacement amplification.

  1. 1. Analysis of Nucleotide Variations in Non-human Primates

    Författare :Ann-Charlotte Rönn; Ann-Christine Syvänen; Mats Nilsson; Phillip Morin; Uppsala universitet; []
    Nyckelord :Molecular genetics; SNP; genotyping; primate; chimpanzee; whole genome amplification; multiple displacement amplification; minisequencing; microarray; Y-chromosome; mitochondria; Genetik;

    Sammanfattning : Many of our closest relatives, the primates, are endangered and could be extinct in a near future. To increase the knowledge of non-human primate genomes, and at the same time acquire information on our own genomic evolution, studies using high-throughput technologies are applied, which raises the demand for large amounts of high quality DNA. LÄS MER

  3. 2. Single-cell population genetics and dispersal limitation of a bloom-forming microalga

    Författare :Raphael Gollnisch; Enhet akvatisk ekologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Gonyostomum semen; algal blooms; invasive species; population structure; RADseq; single-cell whole genome amplification; multiple displacement amplification; calcium; pH; iron;

    Sammanfattning : Microalgal range expansions are increasing in frequency and magnitude worldwide mostly due to anthropogenic factors. However, they often remain unnoticed until mass development (algal blooms) occurs due to difficulties in detection when population densities are low. LÄS MER

  4. 3. Diversity of Skin Infections

    Författare :Johanna Ekström; Malmö Klinisk mikrobiologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Human papillomavirus; high-throughput sequencing; Staphylococcus auereus; multiple displacement amplification; non melanoma skin cancer;

    Sammanfattning : The identification of infectious agents in cancer has been one of the most rewarding endeavors in cancer research. Currently about 20% of the global cancer burden is linked to an infection. A common characteristic of virus-induced cancer is an increased incidence in immunosuppressed patients, presumably because of impaired host control of virus. LÄS MER

  5. 4. Droplet microfluidics for high throughput biological analysis

    Författare :Håkan Jönsson; Helene Andersson-Svahn; Andrew Griffiths; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Microfluidics; Droplet microfluidics; High throughput biology; Single cell analysis; Hydrodynamic separation; Enzyme amplification; Fluorescence polarization; Microfabrication; Bioengineering; Bioteknik; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

    Sammanfattning : Many areas of biological research increasingly perform large-scale analyses.  In genomics the entire gene repertoire of an organism is analyzed.  Proteomics attempts to understand the function and expression patterns of all proteins in a cell or organism. LÄS MER

  7. 5. Using Minisequencing Technology for Analysing Genetic Variation in DNA and RNA

    Författare :Mona Fredriksson; Ann-Christine Syvänen; Aarno Palotie; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Molecular medicine; microarray; minisequencing; molecular medicine; single nucleotide polymorphism; stem cell transplantation; whole genome amplification; allelic imbalance; alternative splicing; Molekylärmedicin; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ;

    Sammanfattning : In this thesis, the four-color fluorescence tag-microarray minisequencing system pioneered by our group was further developed and applied for analysing genetic variation in human DNA and RNA. A SNP marker panel representing different chromosomal regions was established and used for identification of informative SNP markers for monitoring chimerism after stem cell transplantation (SCT). LÄS MER