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Visar resultat 1 - 5 av 9 avhandlingar som matchar ovanstående sökkriterier.

  1. 1. Probing Control : Analysis and Design with Application to Fed-Batch Bioreactors

    Författare :Stéphane Velut; Institutionen för reglerteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Elektronik och elektroteknik; Electronics and Electrical technology; Large scale fermentations; Extremum control; Feeding strategy; Probing control; Fed-batch bioreactors; Automation; robotics; control engineering; Automatiska system; robotteknik; reglerteknik;

    Sammanfattning : In most control problems the objective is to control the output at a desired value in spite of disturbances. In some cases, the best setpoint is not known a priori and it should be found online to optimize the process performance. This thesis examines a probing strategy that can be applied for this class of problems. LÄS MER

  3. 2. Large-scale Bioreactor Phenomena and Characteristics; Effects on E.coli fed batch fermentations on 30m3 scale

    Författare :Gustav Silfversparre; KTH; []
    Nyckelord :Large-scale; E.coli; fed batch; concentration gradients; mixing;

    Sammanfattning : Due to economical and practical limitations the fluiddynamics and mass transport properties are less optimal inlarge-scale bioreactors compared to lab-scale bioreactors. Incombination with the microbial reaction rates this creates amuch more dynamic environment for cells cultivated inlarge-scale bioreactors compared to lab-scale bioreactors. LÄS MER

  4. 3. Impact of glucose uptake rate on recombinant protein production in Escherichia coli

    Författare :Emma Bäcklund; Gen Larsson; Olle Holst; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; AIDA-autotransporter; Escherichia coli; fed-batch; glucose uptake rate; integral membrane proteins; outer membrane proteins; periplasmic retention; phosphotransferase system; recombinant proteins; specific growth rate; surface expression; Biology; Biologi;

    Sammanfattning : Escherichia coli (E. coli) is an attractive host for production of recombinant proteins, since it generally provides a rapid and economical means to achieve high product quantities. LÄS MER

  5. 4. Feeding Strategies Based on Probing Control for E. coli and V. cholerae Cultivations

    Författare :Lena de Maré; Institutionen för reglerteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; robotics; control engineering; Automatiska system; robotteknik; reglerteknik; Automation; Temperature; Mid-ranging control; Amino acid; Complex media; Fed-batch bioreactors; Probing control; Biotechnology; Bioteknik; Parameter estimation; Feeding strategies;

    Sammanfattning : The recombinant DNA techniques have made it possible to produce many different proteins for a wide range of applications. The vector encoding for the recombinant protein is often inserted in the bacterium E. coli as it is a well studied and a well-known organism. To achieve a high productivity, it is important to reach a high cell density. LÄS MER

  7. 5. Innovative Bioreactors for the Degradation of Polycyclic Aromatic Hydrocarbons

    Författare :Benoit Guieysse; Bioteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Environmental technology; Bioteknik; Biotechnology; PAH; degradation; aromatic; contaminant; pollutant; bioreactor; organic pollutant; kontroll av utsläpp; Miljöteknik; pollution control;

    Sammanfattning : The development of biological reactors for the treatment of toxic and recalcitrant organic pollutants is a complex task. Firstly, microbial inoculation, acclimation and selection must be optimized to provide the best microflora possible. LÄS MER