Sökning: "fed batch"

Visar resultat 1 - 5 av 75 avhandlingar innehållade orden fed batch.

1. 1. Metabolic analysis of shikimic acid producing Escherichia coli

   Författare :Louise Johansson; Avdelningen för kemiteknik; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Kemiteknik och kemisk teknologi; Biokemi; metabolism; Chemical technology and engineering; transcriptome analysis; phosphorus-limitation; carbon-limitation; fed-batch; chemostat; batch; fermentation technology; Escherichia coli; shikimic acid; Biotechnology; Bioteknik; Metabolism; Biochemistry; principal component analysis; 2D-fluorescence spectrophotometry; metabolic flux analysis; carbon-rich;

   Sammanfattning : Shikimic acid production using recombinant E.coli has been developed during the past 15 years due to the increased demand of this product, primarily as a starting material for the anti-influensa medicine Tamiflu?. A detailed metabolic analysis of a shikimic acid producing E. LÄS MER

3. 2. Perturbation-based Control of Industrial Fed-batch Bioprocesses

   Författare :Ola Johnsson; Institutionen för reglerteknik; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; perturbation-based control; process control; fed-batch control; industrial bioprocesses;

   Sammanfattning : The topic of this thesis is bioprocess control, more specifically control of industrial-scale microbial fed-batch bioprocesses. Its focus is therefore on methods which are easy to implement in an industrial setting, which gives certain limitations on sensors, actuators and control systems. LÄS MER

4. 3. Development of a protein-free fed-batch process for NS0 cells: studies on regulation of proliferation

   Författare :Erika Spens; Lena Häggström; Jean-Louis Goergen; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NS0 myeloma cells; protein-free medium; fed-batch; conditioned medium; autocrine growth factors; abortive proliferation; protease activity; aprotinin; gp130; Bioengineering; Bioteknik;

   Sammanfattning : The overall objective of this study was to investigate how NS0 cell proliferation is regulated in protein-free media. The hypothesis was that during the adaptation to growth factor-free media, animal cell lines start to produce their own autocrine growth factors to support proliferation, and after some time in a culture the effects of these factors are lost which results in cessation of proliferation. LÄS MER

5. 4. Large-scale Bioreactor Phenomena and Characteristics; Effects on E.coli fed batch fermentations on 30m3 scale

   Författare :Gustav Silfversparre; KTH; []
   Nyckelord :Large-scale; E.coli; fed batch; concentration gradients; mixing;

   Sammanfattning : Due to economical and practical limitations the fluiddynamics and mass transport properties are less optimal inlarge-scale bioreactors compared to lab-scale bioreactors. Incombination with the microbial reaction rates this creates amuch more dynamic environment for cells cultivated inlarge-scale bioreactors compared to lab-scale bioreactors. LÄS MER

7. 5. Probing Control : Analysis and Design with Application to Fed-Batch Bioreactors

   Författare :Stéphane Velut; Institutionen för reglerteknik; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Elektronik och elektroteknik; Electronics and Electrical technology; Large scale fermentations; Extremum control; Feeding strategy; Probing control; Fed-batch bioreactors; Automation; robotics; control engineering; Automatiska system; robotteknik; reglerteknik;

   Sammanfattning : In most control problems the objective is to control the output at a desired value in spite of disturbances. In some cases, the best setpoint is not known a priori and it should be found online to optimize the process performance. This thesis examines a probing strategy that can be applied for this class of problems. LÄS MER