Sökning: "E. coli display"

Visar resultat 6 - 10 av 32 avhandlingar innehållade orden E. coli display.

1. 6. Engineering and applications of surface displayed tyrosinase on Escherichia coli

   Författare :David Hörnström; Antonius J. A. van Maris; Joachim José; KTH; []
   Nyckelord :Bioteknologi; Biotechnology;

   Sammanfattning : The rise of biotechnology has provided a toolbox to deal with major challenges related to pollution and health. Microbial enzymes constitute powerful macromolecules with applications in environmental technology, and industrial and medical production. LÄS MER

3. 7. Bifunctional ADAPTs: Opportunity for serological Half-life extension and Targeted therapy

   Författare :Andreas Wisniewski; Sophia Hober; Masood Kamali-Moghaddam; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ABD; ADAPT; Autoimmune diseases; Cancer; Half-life extension; HRP; Phage Display; Protein Engineering; Targeted therapy; Biotechnology; Bioteknologi;

   Sammanfattning : Small engineered scaffold proteins (ESPs) gain more and more popularity as biological drugs, due to their specificity and applicability in diagnostics and therapy. Thanks to their high stability, low immunogenicity and low production cost, they present themselves as a promising alternative to the market-leading antibodies. LÄS MER

4. 8. Vitreoscilla Haemoglobin - Peroxidase Activity and Metabolic Aspects

   Författare :Malin Kvist; Tillämpad biokemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; metabolism; Biokemi; Metabolism; Biochemistry; Naturvetenskap; Natural science; lipid peroxidation; hypoxia; salt stress; Escherichia coli; Lactococcus lactis; hydrogen-bonding network; proximal site; mutations; Vitreoscilla haemoglobin; peroxidase activity; Proteins; enzymology; Proteiner; enzymologi;

   Sammanfattning : The function of non-vertebrate haemoglobins (Hbs) appears more diverse than that of vertebrate Hbs, possibly reflecting the various demands to be met among their hosts. Sharing structural features with other haem proteins/enzymes, Hbs display a qualitative overlap in activities with functions extending beyond oxygen transport/storage. LÄS MER

5. 9. Rational and combinatorial genetic engineering approaches for improved recombinant protein production and purification

   Författare :Nina Bandmann; Per-Åke Nygren; Helena Berglund; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Aqueous two-phase system; combinatorial library; expression vector; flow cytometry; fusion tag; partitioning; peptide library; phage display; recombinant proteins; ribosomal binding site; translation initiation; Bioengineering; Bioteknik;

   Sammanfattning : The bacterium Escherichia coli (E. coli) is in many situations an ideal host for production of recombinant proteins, since it generally provides a rapid and economical means to achieve sufficiently high product quantities. LÄS MER

7. 10. Synthetic biology approaches for improving production of fatty acid-derived compounds in cyanobacteria

   Författare :Ivana Cengic; Elton P. Hudson; Mathias Uhlén; Annegret Wilde; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; cyanobacteria; metabolic engineering; surface display; CRISPRi; fatty alcohols; fatty acid synthesis; aldehyde deformylating oxygenase; Bioteknologi; Biotechnology;

   Sammanfattning : The environmental consequences associated with the use of fossil-sourced fuels and chemicals have brought with it a realization that future development must move in a more sustainable direction. Currently available biofuels or renewably produced chemical, such as bioethanol or biodiesel, are produced from microbial fermentation of sugar-rich crops or by chemical conversion of natural oils or fats. LÄS MER