Sökning: "Aqueous two-phase system"

Visar resultat 1 - 5 av 30 avhandlingar innehållade orden Aqueous two-phase system.

  1. 1. Peptide-tagged proteins in aqueous two-phase systems

    Författare :Anna Nilsson; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Metabolism; Biokemi; metabolism; Biochemistry; green fluorescent protein; cutinase; tryptophan; Aqueous two-phase system; peptide-tagged protein;

    Sammanfattning : This thesis deals with proteins containing peptide tags for improved partitioning in aqueous two-phase systems. Qualitatively the peptide-tagged protein partitioning could be predicted from peptide data, i.e. partitioning trends found for peptides were also found for the peptide-tagged proteins. LÄS MER

  2. 2. Partitioning of biomolecules in thermoseparating polymer-water two-phase systems

    Författare :Hans-Olof Johansson; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; aqueous two-phase system; temperature-induced phase separation; amino acid and peptide separation; polymer; partitioning; Biology; Biologi;

    Sammanfattning : The partitioning behaviour of different types of molecules in thermoseparating two-phase systems has been studied. A thermoseparating two phase system was formed by heating a water solution, which contained a thermoseparating polymer, above the cloud point temperature. LÄS MER

  3. 3. Integration of Aqueous Two-Phase Systems into Recovery Processes for Biomolecules

    Författare :Cecilia Kepka; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Proteins; proteins; plasmid DNA; large-scale; protein purification; integration; Expression; aqueous two-phase systems; enzymology; Proteiner; enzymologi;

    Sammanfattning : The main scope of this thesis has been to study and develop new purification methods and also to further develop existing methods for purification to enhance their efficacy. Important parameters in the development of the methods are that they should be suitable for scale-up and possible to be integrated into recovery processes. LÄS MER

  4. 4. Separation Methods in Membrane Proteomics -Integration of Aqueous Two-Phase Partitioning for Enrichment and Pre-Fractionation

    Författare :Henrik Everberg; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Membrane proteins; Proteins; metabolism; Biokemi; Metabolism; Biochemistry; Aqueous two-phase partitioning; enzymology; Proteiner; enzymologi; Proteomics;

    Sammanfattning : The study of the protein complement of a genome is called proteomics. Proteomics can be defined as identification, quantification and characterization of a total set of proteins expressed at different time-points or under different cellular states to provide global information about biological processes. LÄS MER

  5. 5. Effects of fusion tags on protein partitioning In aqueous two-phase systems and use in primary protein recovery

    Författare :Cynthia Hassinen; KTH; []
    Nyckelord :E. coli; Aqueous two-phase systems; Fusion proteins; Hydrophobic interaction chromatography; Expanded bed adsorption; Beta-galactosidase; Klenow polymerase; Z-domain; Peptide tags;

    Sammanfattning : The two techniques aqueoustwo-phase partitioning and expanded bed adsorption that bothare suitable for primary protein recovery were studied. Most ofthe work was focused on partition in aqueous two-phase systemsand in particular on the possibility to effect the partitionbehaviour by fusion of short peptide tags or protein domains tothe target protein. LÄS MER